Photoreduction of ferricytochrome c in the presence of potassium ferrocyanide

Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology
Dmitry Malyshka, Reinhard Schweitzer-Stenner

Abstract

Ferricytochrome c has been previously shown to photoreduce in the presence of ferrocyanide anions, but the process has been poorly understood. Here, we propose a kinetic model for this phenomenon and support it with resonance Raman measurements that show a clear dependence of photoreduction on the concentration of mixture components and laser power. Our data are consistent with the photoreduction process that is driven by photoexcitation of the heme followed by an electron transfer from the excited state of the heme macrocycle to the heme iron. Concomitantly, the hole in the heme ground state is filled with an electron transferred from a ferrocyanide ion residing in the heme pocket.

References

Mar 1, 1974·Photochemistry and Photobiology·W P Vorkink, M A Cusanovich
Dec 1, 1981·Biophysical Journal·N Ohno, M A Cusanovich
May 1, 1980·Quarterly Reviews of Biophysics·C DeLisi
Jun 11, 2004·Annual Review of Biochemistry·Xuejun Jiang, Xiaodong Wang
Apr 23, 2014·Proceedings of the National Academy of Sciences of the United States of America·Yuhan SunPaul M Champion
Sep 16, 2015·The Journal of Physical Chemistry. B·Leah A Pandiscia, Reinhard Schweitzer-Stenner

Citations

Related Concepts

Cytochromes c
Heme
Electron Microscopy
Spectrum Analysis, Raman
Hexacyanoferrate II
Potassium ferrocyanide
Macrocyclic Compounds
Heme Iron

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