Photosensitive Alternative Splicing of the Circadian Clock Gene timeless Is Population Specific in a Cold-Adapted Fly, Drosophila montana

G3 : Genes - Genomes - Genetics
Riikka TapanainenMaaria Kankare

Abstract

To function properly, organisms must adjust their physiology, behavior and metabolism in response to a suite of varying environmental conditions. One of the central regulators of these changes is organisms' internal circadian clock, and recent evidence has suggested that the clock genes are also important in the regulation of seasonal adjustments. In particular, thermosensitive splicing of the core clock gene timeless in a cosmopolitan fly, Drosophila melanogaster, has implicated this gene to be involved in thermal adaptation. To further investigate this link we examined the splicing of timeless in a northern malt fly species, Drosophila montana, which can withstand much colder climatic conditions than its southern relative. We studied northern and southern populations from two different continents (North America and Europe) to find out whether and how the splicing of this gene varies in response to different temperatures and day lengths. Interestingly, we found that the expression of timeless splice variants was sensitive to differences in light conditions, and while the flies of all study populations showed a change in the usage of splice variants in constant light compared to LD 22:2, the direction of the shift varied betwee...Continue Reading

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Citations

Jul 17, 2018·Genome Biology and Evolution·Darren J ParkerMichael G Ritchie
Oct 27, 2021·The FEBS Journal·Yao D Cai, Joanna C Chiu

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Datasets Mentioned

BETA
MG279509
MG279527

Methods Mentioned

BETA
Assay
PCR
electrophoresis

Software Mentioned

R Core
tim
CFX Manager
ls
PRANK
R
Sequence Analysis

Related Concepts

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