Photosensitization by antitumor agents, 4. Anthrapyrazole-photosensitized formation of single strand-breaks in DNA

Free Radical Biology & Medicine
J A HartleyJ W Lown

Abstract

Single-strand breaks can be introduced into PM2 closed-circular DNA upon illumination with blue light, in the presence of the anthrapyrazole antitumor agent, compound 1. Damage is observed already after 1 min of blue light illumination, and is significantly enhanced by the presence of electron donors such as NADH, ascorbic acid or Fe(III)/EDTA complex. The photosensitizing properties were not observed for anthrapyrazole analogues with one or more hydroxyl substituents in the chromophore of the drug. The inhibitory effects of sodium azide, methanol, mannitol, SOD, and catalase suggest an oxygen-dependent mechanism of strand-break production, probably involving hydroxyl radicals. However, a second mechanism involving drug molecules bound to the DNA is also indicated under anoxic conditions in the presence of NADH.

References

Jul 25, 1977·Biochemical and Biophysical Research Communications·J Goodman, P Hochstein
Jun 6, 1977·Biochemical and Biophysical Research Communications·J W LownR Y Chang
Dec 1, 1977·Chemico-biological Interactions·W S Thayer
Aug 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·T D Tullius, B A Dombroski
Jun 1, 1986·Biochimie·J CadetP Vigny
Jan 1, 1985·Free Radical Research Communications·A W NieuwintH Joenje
Apr 1, 1974·Journal of the National Cancer Institute·T J Dougherty
Sep 30, 1980·Biochemical and Biophysical Research Communications·G Peters, M A Rodgers
Jan 1, 1983·Molecular and Cellular Biochemistry·J W Lown
Jul 1, 1982·Photochemistry and Photobiology·P J GrayA G Wedd

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Citations

Oct 1, 1991·Pharmacology & Therapeutics·D W Fry
Jan 1, 1994·Pharmacology & Therapeutics·Z Diwu, J W Lown
Jul 1, 1995·Photochemistry and Photobiology·G CondorelliS Sortino
Jan 6, 2011·Journal of Clinical Pharmacy and Therapeutics·N SuhailN Banu
Apr 1, 1990·Biophysical Chemistry·K ReszkaJ W Lown

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