PMID: 11332038May 3, 2001Paper

Photosensitization-induced calcium overload in cardiac cells: direct link to membrane permeabilization and calcium influx

Photochemistry and Photobiology
M TarrD P Valenzeno

Abstract

The purpose of the present study was to gain new insight regarding the role membrane permeabilization plays in the photosensitization-induced increase in intracellular calcium concentration. During continuous rose bengal photosensitization we monitored the contractile state (relaxed or hypercontracted) of isolated frog cardiac cells and assessed the photosensitization-induced membrane-leak conductance. We investigated the effects of irradiance, extracellular calcium concentration, intracellular chelation of calcium and substitution of tetraethylammonium (TEA) for extracellular sodium. We found that with 2 and 5 mM extracellular calcium cell hypercontracture occurred when leak conductance reached values on the order of 6-7 nS, independent of the illumination duration required to reach this conductance. With 0.5 mM calcium hypercontracture occurred when leak conductance reached values on the order of 11 nS. Chelation of intracellular calcium delayed the onset of cell hypercontracture and increased by two- to three-fold the leak conductance at the initiation of cell hypercontracture. Substitution of TEA for extracellular sodium did not affect the time to contracture onset but reduced leak conductance at contracture onset nearly th...Continue Reading

References

Oct 1, 1992·Journal of Molecular and Cellular Cardiology·G YonuschotJ F Novotny
Jun 30, 1992·Biochimica Et Biophysica Acta·L C PenningJ Van Steveninck
Nov 18, 1991·Biochimica Et Biophysica Acta·K G Specht, M A Rodgers
Aug 1, 1991·Photochemistry and Photobiology·E Ben-HurJ Van Steveninck
May 1, 1991·Journal of Molecular and Cellular Cardiology·M Tarr, D P Valenzeno
Feb 1, 1991·Photochemistry and Photobiology·D P Valenzeno, M Tarr
Jun 1, 1986·The Journal of General Physiology·J R Hume, A Uehara
Sep 1, 1988·Journal of Molecular and Cellular Cardiology·L Ver DonckM Borgers
May 1, 1995·Journal of Molecular and Cellular Cardiology·M TarrD Valenzeno
Apr 1, 1994·Free Radical Biology & Medicine·M TarrD P Valenzeno
Jul 1, 1996·Photochemistry and Photobiology·A HubmerB Krammer
Jan 9, 1998·Photochemistry and Photobiology·A C MoorJ VanSteveninck

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Citations

Oct 26, 2005·The Journal of Membrane Biology·G Stark
Sep 23, 2009·IEEE Transactions on Bio-medical Engineering·Arisa ItoTsunenori Arai
Sep 2, 2009·Biophysical Journal·Karin A RiskeRosangela Itri
Oct 31, 2001·Photochemistry and Photobiology·D P Valenzeno, M Tarr
Jun 1, 2007·Lab on a Chip·Norbert KlaukeJonathan M Cooper
Oct 12, 2010·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Thomas BreitenbachJohn D C Lambert

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