Photosensitization of CdSe/ZnS QDs and reliability of assays for reactive oxygen species production

Nanoscale
Daniel R CooperJay L Nadeau

Abstract

CdSe/ZnS quantum dots (QDs) conjugated to biomolecules that can act as electron donors are said to be "photosensitized": that is, they are able to oxidize or reduce molecules whose redox potential lies inside their band edges, in particular molecular oxygen and water. This leads to the formation of reactive oxygen species (ROS) and phototoxicity. In this work, we quantify the generation of different forms of ROS from as-synthesized QDs in toluene; water-solubilized, unconjugated QDs; QDs conjugated to the neurotransmitter dopamine; and dopamine alone. Results of indirect fluorescent ROS assays, both in solution and inside cells, are compared with those of spin-trap electron paramagnetic resonance spectroscopy (EPR). The effect of these particles on the metabolism of mammalian cells is shown to be dependent upon light exposure and proportional to the amount of ROS generated.

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Citations

Jan 18, 2011·Chemical Communications : Chem Comm·Javier Hernández-GilJuan C Mareque-Rivas
Apr 12, 2011·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Vincent MorosiniMuriel Barberi-Heyob
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Jun 20, 2012·Nanotechnology·Xuan ZhangJay Nadeau
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Jan 21, 2010·Environmental Science & Technology·Eve DumasJay L Nadeau
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