Photosensitization of isolated rat liver mitochondria by tetra(m-hydroxyphenyl)chlorin

Archives of Biochemistry and Biophysics
S D KleinC Richter

Abstract

Tetra(m-hydroxyphenyl)chlorin (mTHPC) is used as a photosensitizer in photodynamic therapy (PDT), a novel modality for cancer treatment. Since little is known about mTHPC-mediated damage in vitro, we chose isolated rat liver mitochondria as a model system to study its photodynamic effects. Incubation of isolated mitochondria with mTHPC plus irradiation with light of a wavelength of 652 nm resulted in protein oxidation and lipid peroxidation, as measured by the mitochondrial content of carbonyl groups and thiobarbituric acid-reactive substances, respectively. Type I and type II photochemical reactions contribute to this oxidative damage as shown by the use of scavengers. Photodynamically treated mitochondria had a reduced membrane potential, and their Ca2+ uptake was impaired. Oxygen consumption of complex I of the respiratory chain was stimulated at a low concentration of mTHPC plus irradiation, but decreased at higher concentrations, whereas oxygen consumption at complex II and IV decreased with all mTHPC concentrations offered. No mitochondrial changes were seen with mTHPC in the absence of irradiation. Our results confirm the sensitivity of mitochondria to PDT and may help to understand the mechanisms by which PDT using mTHP...Continue Reading

References

Jan 1, 1978·Methods in Enzymology·J A Buege, S D Aust
Oct 1, 1976·FEBS Letters·K E Akerman, M K Wikström
May 1, 1992·Photochemistry and Photobiology·K W WoodburnD R Phillips
Jan 1, 1992·Photochemistry and Photobiology·B W Henderson, T J Dougherty
Nov 1, 1991·Photochemistry and Photobiology·K W WoodburnD R Phillips
Jan 1, 1990·Methods in Enzymology·R L LevineE R Stadtman
Apr 15, 1990·Journal of Photochemistry and Photobiology. B, Biology·C Salet, G Moreno
Apr 1, 1990·Photochemistry and Photobiology·A W Girotti
Oct 1, 1989·Journal of Photochemistry and Photobiology. B, Biology·A AtlanteC Salet
Aug 30, 1983·Biochemical and Biophysical Research Communications·C SaletF Vinzens
Feb 1, 1981·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·C Salet, G Moreno
Dec 3, 1980·Biochimica Et Biophysica Acta·S Sandberg, I Romslo
Jan 1, 1995·Lasers in Surgery and Medicine·A M FisherC J Gomer
Apr 1, 1995·Journal of Photochemistry and Photobiology. B, Biology·L MorletT Patrice

❮ Previous
Next ❯

Citations

Apr 29, 1999·Clinical Chemistry and Laboratory Medicine : CCLM·V SchlosserH Walt
Aug 19, 2011·Photochemistry and Photobiology·Mathias O Senge, Johan C Brandt
Dec 7, 2011·Human & Experimental Toxicology·A PariharP Ghafourifar
Oct 24, 1998·Archives of Biochemistry and Biophysics·C SaletF Ricchelli
Jan 6, 2000·Archives of Biochemistry and Biophysics·R GalántaiJ Fidy

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.