Photosensitized oxidation and inactivation of pyocyanin, a virulence factor of Pseudomonas aeruginosa

Photochemistry and Photobiology
K ReszkaBradley E Britigan

Abstract

Pyocyanin (PyO-) (1-hydroxy-5-methylphenazine) is a cytotoxic compound secreted by Pseudomonas aeruginosa, an omnipresent bacterium and a human pathogen. We report that visible light illumination in the presence of rose bengal, or riboflavin, in aerated solutions (pH 7.0-7.2) induces irreversible loss of the pigment's characteristic absorption band at 690 nm, indicating its oxidation. This photobleaching was paralleled by generation of a multiline Electron Paramagnetic Resonance (EPR) spectrum attributed to a PyO(-)-derived radical. The reaction was dependent on the presence of air, sensitizers and light, was inhibited by sodium azide and was unaffected by ethanol. This suggests that PyO- was oxidized largely via singlet oxygen and that hydroxyl radicals were not involved. The photochemically modified pigment was less efficient in oxidizing NAD(P)H and generated less superoxide (by approximately 50%) than the intact PyO-, indicating its partial inactivation. 1-Methoxy-5-methylphenazine, a PyO- analog in which the -O- moiety was replaced by the methoxy group (-OMe), was resistant to oxidation, suggesting that oxidation of PyO- involves its phenolate moiety. These results also suggest that photosensitization could be a potentiall...Continue Reading

References

Aug 1, 1979·Journal of General Microbiology·C Propst, L Lubin
Sep 1, 1979·Archives of Biochemistry and Biophysics·H M Hassan, I Fridovich
Aug 1, 1992·British Journal of Pharmacology·R J GryglewskiJ R Vane
Jul 15, 1992·Journal of Photochemistry and Photobiology. B, Biology·Z MalikY Nitzan
Jul 11, 1991·Photochemistry and Photobiology·C M KrishnaD Balasubramanian
Feb 1, 1991·The American Journal of Physiology·J T JackowskiA Wanner
Jul 1, 1989·Journal of Applied Physiology·N C MunroP J Cole
Apr 1, 1986·Infection and Immunity·C D Cox
Mar 1, 1987·Infection and Immunity·K M MillerR U Sorensen
Apr 1, 1985·The Journal of Applied Bacteriology·J G BanksA D Dodge
Jul 1, 1993·Chemical Research in Toxicology·K BrivibaS Steenken
May 1, 1993·Photochemistry and Photobiology·H KimP Riesz
Jan 1, 1997·Clinical Microbiology Reviews·R A Miller, B E Britigan
Jul 1, 1997·Photochemistry and Photobiology·C R Lambert, I E Kochevar
Dec 8, 1998·Journal of Applied Physiology·I T LauredoW M Abraham
Mar 4, 1999·Letters in Applied Microbiology·K F ChilversJ D Perry
Dec 7, 2000·Photochemistry and Photobiology·N KömerikS Poole
Sep 1, 2001·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·M SzpakowskaA Graczyk
Feb 2, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Lynne R UsherMoira K B Whyte
Feb 28, 2002·Current Medicinal Chemistry·Mark Wainwright
Jul 23, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Yunxia Q O'MalleyBradley E Britigan
Nov 8, 2003·Proceedings of the National Academy of Sciences of the United States of America·Huimin RanGee W Lau
Mar 17, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·Yunxia Q O'MalleyBradley E Britigan
May 4, 2004·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Giulio Jori, Stanley B Brown
May 4, 2004·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Michael R Hamblin, Tayyaba Hasan
May 12, 2004·Free Radical Biology & Medicine·Krzysztof J ReszkaBradley E Britigan
Jun 12, 2004·Photochemistry and Photobiology·Walter A MassadNorman A Garcia
Jul 13, 2004·Journal of Photochemistry and Photobiology. B, Biology·Chia-Fen LeeChing-Tsan Huang
Oct 14, 2004·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Tim MaischChristoph Abels
Nov 30, 2004·Trends in Molecular Medicine·Gee W LauFansheng Kong
May 12, 2005·Current Microbiology·Mrinalini SharmaPradeep Kumar Gupta
Jun 14, 2005·Trends in Microbiology·Gee W LauBradley E Britigan
Feb 1, 1997·International Journal of Antimicrobial Agents·M SzpakowskaJ Grzybowski

❮ Previous
Next ❯

Citations

Oct 16, 2008·Biotechnology Letters·Pattanathu K S M RahmanZulfiqur Ali
Aug 5, 2008·Photochemistry and Photobiology·Cale N StreetNicolas G Loebel
Apr 23, 2010·Photochemistry and Photobiology·Krzysztof J ReszkaBradley E Britigan
Mar 13, 2012·Photochemistry and Photobiology·Maria C E HashimotoMartha S Ribeiro
Nov 13, 2008·Photodermatology, Photoimmunology & Photomedicine·Eram Husain, Imrana Naseem
Sep 6, 2011·Redox Report : Communications in Free Radical Research·D M KasoziKatja Becker
Dec 25, 2019·Lasers in Medical Science·Heather DurkeeJean-Marie Parel

❮ Previous
Next ❯

Software Mentioned

WinSim

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2022 Meta ULC. All rights reserved