Photosensitized oxidation of phosphatidylethanolamines monitored by electrospray tandem mass spectrometry

Journal of Mass Spectrometry : JMS
Tânia MeloM Rosario M Domingues

Abstract

Photodynamic therapy combines visible light and a photosensitizer (PS) in the presence of molecular oxygen to generate reactive oxygen species able to modify biological structures such as phospholipids. Phosphatidylethanolamines (PEs), being major phospholipid constituents of mammalian cells and membranes of Gram-negative bacteria, are potential targets of photosensitization. In this work, the oxidative modifications induced by white light in combination with cationic porphyrins (Tri-Py(+)-Me-PF and Tetra-Py(+)-Me) were evaluated on PE standards. Electrospray ionization mass spectrometry (ESI-MS) and tandem mass spectrometry (ESI-MS/MS) were used to identify and characterize the oxidative modifications induced in PEs (POPE: PE 16:0/18:1, PLPE: PE 16:0/18:2, PAPE: PE 16:0/20:4). Photo-oxidation products of POPE, PLPE and PAPE as hydroxy, hydroperoxy and keteno derivatives and products due to oxidation in ethanolamine polar head were identified. Hydroperoxy-PEs were found to be the major photo-oxidation products. Quantification of hydroperoxides (PE-OOH) allowed differentiating the potential effect in photodamage of the two porphyrins. The highest amounts of PE-OOH were notorious in the presence of Tri-Py(+)-Me-PF, a highly effic...Continue Reading

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Citations

Feb 20, 2014·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·M A PereiraA Almeida
Mar 26, 2016·Frontiers in Microbiology·Maria BartolomeuAdelaide Almeida
Mar 7, 2017·Future Medicinal Chemistry·Lúcia MarcielAdelaide Almeida
Jan 1, 2019·Mass Spectrometry Reviews·Simone ColomboM Rosário Domingues
Jul 13, 2018·Frontiers in Microbiology·Xiaoqing HuMichael R Hamblin
Dec 29, 2017·Journal of Mass Spectrometry : JMS·Simone ColomboM Rosário Domingues
Feb 9, 2021·Frontiers in Endocrinology·Florian GruberMarkus Schosserer
Jul 11, 2018·Journal of the American Chemical Society·Isabel O L BacellarMauricio S Baptista

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