Photoshop-based image analysis of canine articular cartilage after subchondral damage

Archives of Orthopaedic and Trauma Surgery
A LahmJ Haberstroh

Abstract

The validity of histopathological grading is a major problem in the assessment of articular cartilage. Calculating the cumulative strength of signal intensity of different stains gives information regarding the amount of proteoglycan, glycoproteins, etc. Using this system, we examined the medium-term effect of subchondral lesions on initially healthy articular cartilage. After cadaver studies, an animal model was created to produce pure subchondral damage without affecting the articular cartilage in 12 beagle dogs under MRI control. Quantification of the different stains was provided using a Photoshop-based image analysis (pixel analysis) with the histogram command 6 months after subchondral trauma. FLASH 3D sequences revealed intact cartilage after impact in all cases. The best detection of subchondral fractures was achieved with fat-suppressed TIRM sequences. Semiquantitative image analysis showed changes in proteoglycan and glycoprotein quantities in 9 of 12 samples that had not shown any evidence of damage during the initial examination. Correlation analysis showed a loss of the physiological distribution of proteoglycans and glycoproteins in the different zones of articular cartilage. Currently available software programs ...Continue Reading

Citations

Mar 25, 2010·International Urogynecology Journal·Okechukwu A IbeanuThomas E Nolan
Dec 7, 2005·Archives of Orthopaedic and Trauma Surgery·A LahmD Maier
Oct 10, 2006·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Seppo RönkköHannu Uusitalo
Jan 15, 2013·The Journal of Investigative Dermatology·Ananya Datta MitraSmriti Kundu-Raychaudhuri
Oct 15, 2014·Acta Ophthalmologica·Minna Helin-ToiviainenHannu Uusitalo
Aug 24, 2010·American Journal of Ophthalmology·Srinivas AmbatipudiK V Chalam
Dec 11, 2014·World Journal of Clinical Oncology·Xin ChenXiao-Zhi Zhang
Dec 7, 2007·International Journal of Radiation Biology·Kumari L AndarawewaMary Helen Barcellos-Hoff
Aug 3, 2019·Cartilage·Sonja M WalzerReinhard Windhager
Dec 20, 2007·Applied Immunohistochemistry & Molecular Morphology : AIMM·Luciano Serpa HammesJoão Sabino Cunha-Filho
Apr 23, 2013·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Tobias EckleHolger K Eltzschig
Dec 31, 2005·The Journal of Clinical Endocrinology and Metabolism·Mei-Ju ShihLee-Ming Chuang
Jan 24, 2007·Proceedings of the Institution of Mechanical Engineers. Part H, Journal of Engineering in Medicine·G P StachowiakP Podsiadlo

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