PMID: 11607335Nov 1, 1992Paper

Photosynthetic membrane topography: quantitative in situ localization of photosystems I and II

Proceedings of the National Academy of Sciences of the United States of America
L MustardyE Gantt

Abstract

An immunolabeling approach was developed for quantitative in situ labeling of photosystems I and II (PSI and PSII). Photosynthetic membranes from the phycobilisome-containing red alga Porphyridium cruentum were isolated from cells in which different photosystem compositions were predetermined by growing cells in green light (GL) or red light (RL). Based on phycobilisome densities per membrane area of 390 per m2 (GL) and 450 per m2 (RL) and the PSI reaction center (P700) and PSII reaction center (QA) content, the photosystem densities per m2 of membrane were calculated to be 2520 PSI in GL and 1580 in RL and 630 PSII in GL and 1890 in RL. PSI was detected in the membranes with 10-nm Au particles conjugated to affinity-purified anti-PSI, and PSII was detected with 15-nm Au particles conjugated to anti-PSII. Distribution of Au particles appeared relatively uniform, and the degree of labeling was consistent with the calculated photosystem densities. However, the absolute numbers of Au-labeled sites were lower than would be obtained if all reaction center monomers were labeled. Specific labeling of PSI was 25% in GL and RL membranes, and PSII labeling was 33% in GL but only 17% in RL membranes. An IgG-Au particle is larger than a mo...Continue Reading

References

Apr 1, 1991·Trends in Biochemical Sciences·J Lavergne, P Joliot
Jun 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·O AlmogR Nechushtai
Oct 1, 1989·The Journal of Cell Biology·J E Hinshaw, K R Miller
Dec 1, 1988·European Journal of Biochemistry·K D IrrgangG Renger

❮ Previous
Next ❯

Citations

Feb 8, 1994·Biochimica Et Biophysica Acta·S Jansson
Nov 20, 2012·Annual Review of Plant Biology·Elisabeth Gantt
Jan 2, 2021·Journal of Plant Physiology·John A Raven

❮ Previous
Next ❯

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.