Photosynthetic performance and fertility are repressed in GmAOX2b antisense soybean.

Plant Physiology
Tsun-Thai ChaiPatrick M Finnegan

Abstract

The alternative oxidase (AOX) is a cyanide-resistant oxidase that provides an alternative outlet for electrons from the respiratory electron transport chain embedded in the inner membrane of plant mitochondria. Examination of soybean (Glycine max) plants carrying a GmAOX2b antisense gene showed AOX to have a central role in reproductive development and fecundity. In three independently transformed antisense lines, seed set was reduced by 16% to 43%, whereas ovule abortion increased by 1.2- to 1.7-fold when compared with nontransgenic transformation control plants. Reduced fecundity was associated with reductions in whole leaf cyanide-resistant, salicylhydroxamic acid-sensitive respiration and net photosynthesis, but there was no change in total respiration in the dark. The frequency of potential fertilization events was reduced by at least one-third in the antisense plants as a likely consequence of prefertilization defects. Pistils of the antisense plants contained a higher proportion of immature-sized, nonfertile embryo sacs compared with nontransgenic control plants. Increased rates of pollen abortion in vivo and reduced rates of pollen germination in vitro suggested that the antisense gene compromised pollen development and...Continue Reading

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Citations

Feb 25, 2014·Plant Physiology and Biochemistry : PPB·Abhaypratap VishwakarmaKollipara Padmasree
Dec 9, 2014·Plant Signaling & Behavior·Jennifer Selinski, Renate Scheibe
Sep 1, 2016·The New Phytologist·Keshav Dahal, Greg C Vanlerberghe
Feb 10, 2018·Plant, Cell & Environment·Crystal SweetmanDavid A Day
May 16, 2020·The Plant Journal : for Cell and Molecular Biology·Monica Borghi, Alisdair R Fernie
Jul 20, 2020·The Plant Journal : for Cell and Molecular Biology·Yu-Ting LiZi-Shan Zhang
Jun 6, 2020·Frontiers in Plant Science·Jayamini JayawardhaneAbir U Igamberdiev

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