Photosystem activity and state transitions of the photosynthetic apparatus in cyanobacterium Synechocystis PCC 6803 mutants with different redox state of the plastoquinone pool

Biochemistry. Biokhimii︠a︡
Y V BolychevtsevaN V Karapetyan

Abstract

To better understand how photosystem (PS) activity is regulated during state transitions in cyanobacteria, we studied photosynthetic parameters of photosystem II (PSII) and photosystem I (PSI) in Synechocystis PCC 6803 wild type (WT) and its mutants deficient in oxidases (Ox(-)) or succinate dehydrogenase (SDH(-)). Dark-adapted Ox(-) mutant, lacking the oxidation agents, is expected to have a reduced PQ pool, while in SDH(-) mutant the PQ pool after dark adaptation will be more oxidized due to partial inhibition of the respiratory chain electron carriers. In this work, we tested the hypothesis that control of balance between linear and cyclic electron transport by the redox state of the PQ pool will affect PSII photosynthetic activity during state transition. We found that the PQ pool was reduced in Ox(-) mutant, but oxidized in SDH(-) mutant after prolonged dark adaptation, indicating different states of the photosynthetic apparatus in these mutants. Analysis of variable fluorescence and 77K fluorescence spectra revealed that the WT and SDH(-) mutant were in State 1 after dark adaptation, while the Ox(-) mutant was in State 2. State 2 was characterized by ~1.5 time lower photochemical activity of PSII, as well as high rate of ...Continue Reading

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Citations

Jan 7, 2017·Doklady. Biochemistry and Biophysics·I V ElanskayaI N Stadnichuk
Mar 13, 2020·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Pablo I Calzadilla, Diana Kirilovsky
Nov 22, 2018·Frontiers in Plant Science·Ginga Shimakawa, Chikahiro Miyake

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