Photosystem I light-harvesting complex Lhca4 adopts multiple conformations: Red forms and excited-state quenching are mutually exclusive

Biochimica Et Biophysica Acta
Francesca PassariniR Croce

Abstract

In this work we have investigated the origin of the multi-exponential fluorescence decay and of the short excited-state lifetime of Lhca4. Lhca4 is the antenna complex of Photosystem I which accommodates the red-most chlorophyll forms and it has been proposed that these chlorophylls can play a role in fluorescence quenching. Here we have compared the fluorescence decay of Lhca4 with that of several Lhca4 mutants that are affected in their red form content. The results show that neither the multi-exponentiality of the decay nor the fluorescence quenching is due to the red forms. The data indicate that Lhca4 exists in multiple conformations. The presence of the red forms, which are very sensitive to changes in the environment, allows to spectrally resolve the different conformations: a "blue" conformation with a short lifetime and a "red" one with a long lifetime. This finding strongly supports the idea that the members of the Lhc family are able to adopt different conformations associated with their light-harvesting and photoprotective roles. The ratio between the conformations is modified by the substitution of lutein by violaxanthin. Finally, it is demonstrated that the red forms cannot be present in the quenched conformation.

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Citations

Oct 18, 2011·Photosynthesis Research·Erica BelgioRobert Jennings
Aug 3, 2011·Proceedings of the National Academy of Sciences of the United States of America·Tjaart P J KrügerRienk van Grondelle
May 7, 2013·Photosynthesis Research·Roberta Croce, Herbert van Amerongen
Mar 13, 2012·Biochimica Et Biophysica Acta·Emilie WientjesRoberta Croce
Feb 15, 2015·Biochimica Et Biophysica Acta·Clotilde Le QuiniouRoberta Croce
Mar 1, 2011·Biophysical Journal·Emilie WientjesRoberta Croce
Apr 2, 2013·Biochimica Et Biophysica Acta·Robert C JenningsStefano Santabarbara
Jun 28, 2011·Biochimica Et Biophysica Acta·Matteo BallottariRoberto Bassi
May 30, 2014·Proceedings of the National Academy of Sciences of the United States of America·Matteo BallottariRoberto Bassi
Mar 27, 2015·Nature Communications·M YokonoA Tanaka
Jul 30, 2017·Photosynthesis Research·Reza Ranjbar ChoubehHerbert van Amerongen
Sep 3, 2020·Chemical Science·Vincenzo MascoliRoberta Croce
Nov 5, 2020·Physical Chemistry Chemical Physics : PCCP·Martijn TrosElisabet Romero
Oct 18, 2020·Journal of Experimental Botany·Vasco Giovagnetti, Alexander V Ruban
Nov 12, 2018·Biochimica Et Biophysica Acta. Bioenergetics·David BínaTomáš Polívka

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