Phototherapy promotes cell migration in the presence of hydroxyurea.

Lasers in Medical Science
I L ZunguHeidi Abrahamse

Abstract

Phototherapy has been shown to cause an increase in cell proliferation and migration. This study focused on viability (trypan blue), proliferation [sodium 3'-(1-(phenylaminocarbonyl)-3,4-tetrazolium)-bis(4-methoxy-6-nitro)-benzene sulphonic acid hydrate (XTT) and adenosine triphosphate (ATP)] and migration of WS1 cells following irradiation in the presence of hydroxyurea (HU), which is an inhibitor of proliferation. Wounded cells were irradiated on days 1 and 4 with a fluence of 5 J/cm(2) with a helium-neon (He-Ne) laser at 632.8 nm. After a repair time of 24 h, cellular responses were assessed. Wounded irradiated cells without HU showed an increase in cell viability and proliferation, which was confirmed by complete wound closure by day 4. Although wounded irradiated cells treated with 5 mM HU showed incomplete wound closure, these cells showed increased migration compared with that of control cells. This study showed that laser irradiation using an He-Ne laser with a fluence of 5 J/cm(2) stimulates cell viability. The HU results confirmed that laser irradiation promotes cell migration and proliferation.

References

Jun 12, 1999·Journal of Photochemistry and Photobiology. B, Biology·T Karu
Aug 10, 2000·Journal of Neuroscience Research·Z KörnyeiE Madarász
Mar 27, 2001·Methods in Cell Science : an Official Journal of the Society for in Vitro Biology·G CaiD A Beacham
Mar 26, 2003·The Journal of Veterinary Medical Science·Masanobu HayashiToyo Okui
Apr 26, 2003·The Journal of Investigative Dermatology·Xue-Jun ZhangWei Huang
Oct 12, 2004·IEEE Transactions on Nanobioscience·D C WalkerJ Southgate
Jun 16, 2005·Photomedicine and Laser Surgery·D Hawkins, H Abrahamse
May 16, 2006·Lasers in Medical Science·Luciane Hiramatsu AzevedoMárcia Martins Marques
May 19, 2007·Photomedicine and Laser Surgery·N Houreld, H Abrahamse

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