Photothermal cellular stimulation in functional bio-polymer interfaces

Scientific Reports
Nicola MartinoMaria Rosa Antognazza

Abstract

Hybrid interfaces between organic semiconductors and living tissues represent a new tool for in-vitro and in-vivo applications, bearing a huge potential, from basic researches to clinical applications. In particular, light sensitive conjugated polymers can be exploited as a new approach for optical modulation of cellular activity. In this work we focus on light-induced changes in the membrane potential of Human Embryonic Kidney (HEK-293) cells grown on top of a poly(3-hexylthiophene) (P3HT) thin film. On top of a capacitive charging of the polymer interface, we identify and fully characterize two concomitant mechanisms, leading to membrane depolarization and hyperpolarisation, both mediated by a thermal effect. Our results can be usefully exploited in the creation of a new platform for light-controlled cell manipulation, with possible applications in neuroscience and medicine.

References

Aug 2, 2001·Progress in Brain Research·S M Potter
Mar 26, 2005·Optics Letters·Jonathon WellsAnita Mahadevan-Jansen
May 3, 2005·Journal of Pharmacological and Toxicological Methods·Philip Thomas, Trevor G Smart
May 29, 2007·Biophysical Journal·Jonathon WellsE Duco Jansen
Jan 15, 2008·Biophysical Journal·Agnella D IzzoClaus-Peter Richter
Feb 9, 2008·Neuroscience Letters·Peter J ReeceGlen A Cottrell
Oct 16, 2009·Nature·Massimo Scanziani, Michael Häusser
Jan 27, 2011·Nature Communications·Diego GhezziGuglielmo Lanzani
Mar 23, 2011·Nature Photonics·M W JenkinsA M Rollins
Sep 29, 2011·Journal of the American Chemical Society·Vini GautamK S Narayan
Mar 15, 2012·Nature Communications·Mikhail G ShapiroFrancisco Bezanilla
Mar 24, 2012·Journal of Neurophysiology·E S AlbertC Chabbert
Mar 14, 2013·Nature Communications·Dion KhodagholyGeorge G Malliaras
Aug 2, 2013·Journal of Neural Engineering·Nairouz FarahShy Shoham
Sep 7, 2013·Scientific Reports·Austin R DukeE Duco Jansen
Sep 1, 2012·AIP Advances·Benjamin MiglioriWilliam Kristan
Apr 26, 2014·Advanced Materials·Vini GautamK S Narayan
May 30, 2014·International Journal of Nanomedicine·Lilach Bareket-Keren, Yael Hanein
Jun 25, 2014·Lab on a Chip·Lars FaxälvMagnus Berggren
May 1, 2013·Nature Photonics·Diego GhezziFabio Benfenati
Aug 21, 2013·Journal of Materials Chemistry. B, Materials for Biology and Medicine·N MartinoM R Antognazza

❮ Previous
Next ❯

Citations

Apr 6, 2016·Frontiers in Neuroscience·Elisabetta ColomboFabio Benfenati
Sep 9, 2015·Frontiers in Neuroscience·Diego Ghezzi
Aug 3, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Oliya S AbdullaevaManuela Schiek
Mar 5, 2016·Scientific Reports·Paul FeyenDiego Ghezzi
Feb 21, 2018·Advanced Materials·Giorgio E BonacchiniMario Caironi
Apr 18, 2018·Chemical Society Reviews·Francesca Di MariaGuglielmo Lanzani
May 3, 2018·Advanced Materials·David RandEric Daniel Głowacki
May 2, 2018·Chembiochem : a European Journal of Chemical Biology·Ilaria BargigiaGuglielmo Lanzani
Sep 18, 2018·Advanced Materials·Sebastiano BellaniFrancesco Bonaccorso
Feb 28, 2018·Lab Animal·Fabio Benfenati, Guglielmo Lanzani
Mar 13, 2019·Journal of Biophotonics·Qingling Xia, Tobias Nyberg
May 31, 2016·Advanced Healthcare Materials·Maria Rosa AntognazzaFabio Benfenati
Jul 25, 2017·Nature Communications·Mykhailo SytnykEric Daniel Głowacki
Nov 12, 2017·Nature Communications·Michael PlaksinShy Shoham
Jun 9, 2019·Annual Review of Physical Chemistry·Giovanni ManfrediGuglielmo Lanzani
Oct 28, 2019·Nature Reviews. Materials·Yuanwen Jiang, Bozhi Tian
Jul 8, 2020·Proceedings of the National Academy of Sciences of the United States of America·Felix S AlfonsoBianxiao Cui
Aug 19, 2017·Journal of Neural Engineering·Y WeisslerS Shoham
Aug 15, 2018·International Journal of Molecular Sciences·Krishna FeronPaul C Dastoor
Nov 27, 2018·Frontiers in Bioengineering and Biotechnology·Giuseppina PolinoFrancesca Santoro
Apr 12, 2019·Science Advances·Marie JakešováEric Daniel Głowacki
Aug 21, 2015·Journal of Materials Chemistry. B, Materials for Biology and Medicine·S BellaniM R Antognazza
Jul 1, 2020·Nature Nanotechnology·José Fernando Maya-VetencourtFabio Benfenati
Aug 17, 2016·Journal of Neural Engineering·Sohmyung HaGert Cauwenberghs
Aug 21, 2016·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Susana VaqueroMaria Rosa Antognazza
Jul 11, 2020·Advanced Biosystems·Jacky Fong-Chuen LooYi-Ping Ho
Jul 8, 2020·International Journal of Molecular Sciences·Sofia MunicoyMartin F Desimone
Feb 6, 2020·Nature Nanotechnology·Mattia Lorenzo DiFrancescoFabio Benfenati
Mar 15, 2020·Physical Review. E·Aymar C L de LichterveldeMartin Z Bazant
Sep 14, 2018·Frontiers in Bioengineering and Biotechnology·Caterina BossioMaria Rosa Antognazza
Jan 21, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Elena ZucchettiMaria Rosa Antognazza
Nov 17, 2020·Frontiers in Bioengineering and Biotechnology·José Fernando Maya-VetencourtFabio Benfenati
Feb 9, 2021·Frontiers in Physiology·Francesco MocciaFrancesco Lodola
Mar 9, 2021·Neurochemistry International·Mukta AgrawalAmit Alexander
May 14, 2021·ACS Applied Materials & Interfaces·Frano MilosMaria Rosa Antognazza
Jul 13, 2021·Frontiers in Neuroscience·Onuralp KaratumSedat Nizamoglu
Dec 28, 2018·ACS Chemical Neuroscience·Joao L Carvalho-de-SouzaJames B Delehanty
Feb 25, 2017·Accounts of Chemical Research·Nils E PerssonElsa Reichmanis
Jul 1, 2020·ACS Nano·Ratka DamnjanovicVenkat R Bhethanabotla

❮ Previous
Next ❯

Methods Mentioned

BETA
fluorescence microscopy

Software Mentioned

Matlab
pClamp
Image J

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.