Photothermal detection of nicotine-induced apoptotic effects in pancreatic cancer cells

Life Sciences
Valentin GalitovskyVladimir P Zharov

Abstract

The objective of this study was to evaluate the capability of a photothermal (PT) assay in determining the effects of graded doses of nicotine in a pancreatic acinar cell line (AR42J). The cellular response to nicotine was detected through the monitoring of PT signals from light-absorbing endogenous cellular structures that have been used as natural indicators for nicotine's action. It was demonstrated that introducing nicotine to cultured acinar cells in vitro leads to changes in cellular absorbing structures, thereby altering the microstructure of PT cell images and the temporal shape of PT signals. The results showed that the dependence of specific PT parameters was almost proportional to nicotine concentrations ranging from 1 nM to 100 microM, with the saturation maximum at and around 100 microM - 1 mM; thereafter, PT signals decreased rapidly to control levels and even lower, in the range of 1 - 50 mM. Conventional fluorescent tests (Annexin V--Propidium Iodide) performed in parallel showed no effect with nicotine at a concentration <1 microM (three orders of magnitude greater than the sensitivity threshold of the PT assay). With an increase in nicotine concentration from 1 mM to 50 mM, rapidly growing apoptotic and necrot...Continue Reading

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Citations

Oct 10, 2006·Mund-, Kiefer- und Gesichtschirurgie : MKG·M P SemmlerN H Kleinsasser
Sep 12, 2007·Apoptosis : an International Journal on Programmed Cell Death·Reinhard ZeidlerStephan Lang
Oct 24, 2009·Photosynthesis Research·Richard CisekVirginijus Barzda
Jan 2, 2007·Pancreas·Halina MilnerowiczStanislaw Milnerowicz
Jan 11, 2007·Journal of Biomedical Optics·Vladimir P ZharovTimothy C Chambers
Sep 13, 2005·Alcoholism, Clinical and Experimental Research·Nassima Ait-DaoudBankole A Johnson
Jan 13, 2006·The American Journal of Gastroenterology·Uwe A WittelSurinder K Batra

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis