Photothermal Microscopy: Imaging the Optical Absorption of Single Nanoparticles and Single Molecules.

ACS Nano
Subhasis AdhikariMichel Orrit

Abstract

The photothermal (PT) signal arises from slight changes of the index of refraction in a sample due to absorption of a heating light beam. Refractive index changes are measured with a second probing beam, usually of a different color. In the past two decades, this all-optical detection method has reached the sensitivity of single particles and single molecules, which gave birth to original applications in material science and biology. PT microscopy enables shot-noise-limited detection of individual nanoabsorbers among strong scatterers and circumvents many of the limitations of fluorescence-based detection. This review describes the theoretical basis of PT microscopy, the methodological developments that improved its sensitivity toward single-nanoparticle and single-molecule imaging, and a vast number of applications to single-nanoparticle imaging and tracking in material science and in cellular biology.

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Citations

Mar 16, 2021·Optics Letters·Jun JiangMartin C Fischer
Jul 17, 2021·Journal of the American Chemical Society·Minghe LiGarth J Simpson
Aug 3, 2021·Acta Biomaterialia·Eleana ManousiouthakisChristine E Schmidt

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Methods Mentioned

BETA
fluorescence correlation spectroscopy
light scattering
fluorescence microscopy
Circular dichroism
photothermal correlation spectroscopy
fluorescence correlation
FCS
imaging technique
surface plasmon resonance
electron beam lithography

Software Mentioned

iSCAT
PAINT
PIC

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