Photothermal therapy using folate conjugated gold nanoparticles enhances the effects of 6MV X-ray on mouth epidermal carcinoma cells

Journal of Photochemistry and Photobiology. B, Biology
Ali NeshastehrizAli Shakeri-Zadeh

Abstract

The aim of this study was to develop an optimized method for preparation of folate conjugated gold nanoparticles (F-AuNPs) and to investigate its cytotoxic effects and cell apoptosis in combination with photothermal therapy (PTT) and radiotherapy (RT) for the treatment of mouth epidermal carcinoma cells KB. For this purpose, cells were treated with synthesized F-AuNPs at different concentrations for 6h and then irradiated them with laser beam (532nm, 0.5W/cm(2), 15min). After photothermal therapy, the cells were exposed to 6MV X-ray with a single dose of 2Gy. MTT assay were performed to evaluate the cell survival rate and apoptosis was determined by flow cytometry using an annexin V-fluorescein isothiocyanate/propidium iodide apoptosis detection kit. No significant cell damage or cell apoptosis from the individual treatment of laser light or F-AuNPs was observed, while viability of cells incubated with F-AuNPs and then exposed to the laser was significantly decreased. Additionally, our results demonstrated that F-AuNPs is good radiosensitizers even at a low concentration such as 20μM when megavoltage X-ray is used. Also, when KB cells were treated with F-AuNPs under both laser and X-ray irradiation, the cell viability substanti...Continue Reading

Citations

Jan 30, 2018·Chemistry, an Asian Journal·Yang LiuZhigang Xie
Aug 30, 2018·Advanced Materials·Jiani XieYuliang Zhao
Jan 3, 2018·Artificial Cells, Nanomedicine, and Biotechnology·Mehri MirrahimiAli Shakeri-Zadeh
Mar 1, 2018·Artificial Cells, Nanomedicine, and Biotechnology·Elham ZeinizadeS Kamran Kamrava
Oct 10, 2018·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·M MirrahimiS M A Hosseini Nami
Oct 11, 2017·Artificial Cells, Nanomedicine, and Biotechnology·Habib GhaznaviAlireza Montazerabadi
Sep 1, 2019·Journal of Controlled Release : Official Journal of the Controlled Release Society·Majid SharifiMojtaba Falahati
Aug 29, 2021·Pharmaceuticals·Gustavo MoraesWalter L Siqueira

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