Photothermally induced local dissociation of collagens for harvesting of cell sheets

Angewandte Chemie
Jae Dong KimEunkyoung Kim

Abstract

The local heating of poly(3,4-ethylenedioxythiophene) (PEDOT) by a photothermal effect directed by near-infrared (NIR) light induces unfolding of absorbed collagen triple helices, yielding soluble collagen single-helical structures. This dissociation of collagens allowed the harvesting of a living idiomorphic cell sheet, achieved upon irradiation with NIR light (λ=808 nm). The PEDOT layer was patterned and cells were successfully cultured on the patterned substrate. Cell sheets of various shapes mirroring the PEDOT pattern could be detached after a few minutes of irradiation with NIR light. The PEDOT patterns guided not only the entire shape of the cell sheets but also the spreading direction of the cells in the sheets. This photothermally induced dissociation of collagen provided a fast non-invasive harvesting method and tailor-made cell-sheet patterns.

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Citations

Oct 5, 2018·Chemical Society Reviews·Wen LiXiaogang Qu
May 1, 2019·Advanced Biosystems·Jiange JingQinghua Lu
Sep 28, 2016·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Yu Bin LeeHeungsoo Shin
Nov 7, 2019·Advanced Materials·Minsu HanEunkyoung Kim
Jan 7, 2021·International Journal of Molecular Sciences·Chikahiro Imashiro, Tatsuya Shimizu
May 30, 2021·Advanced Materials·Hee Jung KimEunkyoung Kim
Aug 12, 2019·ACS Biomaterials Science & Engineering·Miyeon NohNathaniel S Hwang
Sep 1, 2021·ACS Applied Materials & Interfaces·Youfa LiuFengxing Jiang

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