Phototoxic effects of lysosome-associated genetically encoded photosensitizer KillerRed

Journal of Biomedical Optics
Ekaterina O SerebrovskayaKonstantin A Lukyanov

Abstract

KillerRed is a unique phototoxic red fluorescent protein that can be used to induce local oxidative stress by green-orange light illumination. Here we studied phototoxicity of KillerRed targeted to cytoplasmic surface of lysosomes via fusion with Rab7, a small GTPase that is known to be attached to membranes of late endosomes and lysosomes. It was found that lysosome-associated KillerRed ensures efficient light-induced cell death similar to previously reported mitochondria- and plasma membrane-localized KillerRed. Inhibitory analysis demonstrated that lysosomal cathepsins play an important role in the manifestation of KillerRed-Rab7 phototoxicity. Time-lapse monitoring of cell morphology, membrane integrity, and nuclei shape allowed us to conclude that KillerRed-Rab7-mediated cell death occurs via necrosis at high light intensity or via apoptosis at lower light intensity. Potentially, KillerRed-Rab7 can be used as an optogenetic tool to direct target cell populations to either apoptosis or necrosis.

Citations

Sep 4, 2015·International Journal of Molecular Sciences·Isabel O L BacellarMauricio S Baptista
Mar 2, 2016·Journal of Photochemistry and Photobiology. B, Biology·Francesca BaldassarreGiuseppe Ciccarella
Aug 17, 2016·BioTechniques·Alina P RyuminaKonstantin A Lukyanov
Dec 19, 2015·PloS One·Karen S SarkisyanKonstantin A Lukyanov
Apr 20, 2017·Frontiers in Physiology·Hong Ning JiangZong Jie Cui
Feb 11, 2016·The Biochemical Journal·Heidi Abrahamse, Michael R Hamblin
Jul 6, 2014·Current Protocols in Cytometry·Timothy S Jarvela, Adam D Linstedt
Oct 12, 2018·Scientific Reports·Stephan EndresThomas Drepper
Nov 10, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Suman DasDhermendra K Tiwari
Dec 8, 2020·Frontiers in Chemistry·Pingping LiangMarcel P Bruchez
Jun 3, 2021·International Journal of Molecular Sciences·Olga ShilovaSergey Deyev

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