Phthalate Exposures and MicroRNA Expression in Uterine Fibroids: The FORGE Study

Epigenetics Insights
Ami R ZotaGaby N Moawad

Abstract

Phthalates are associated with multiple, adverse reproductive outcomes including increased risk of uterine leiomyoma (fibroids). Phthalates can interact with epigenetic modifications including microRNAs (miRNAs), which help regulate processes crucial to fibroid pathogenesis. However, no prior study has examined the influence of phthalates on miRNA expression in fibroid tumors. We conducted a preliminary, cross-sectional study to examine the associations between phthalate exposures and miRNA expression levels in fibroid tumors and to explore potential effect modification by race/ethnicity. We quantified expression levels of 754 miRNAs in fibroid tumor samples and analyzed spot urine samples for phthalate metabolites collected from 45 pre-menopausal women undergoing surgery for fibroid treatment at an academic hospital. Associations between miRNA levels in fibroids and phthalate biomarkers were evaluated using linear regression adjusting for age, race/ethnicity, and body mass index (BMI). Statistical tests were adjusted for multiple comparisons. We also performed in silico Ingenuity Pathway Analysis to identify the biological pathways that are regulated by phthalate-associated miRNAs. Mono-hydroxybutyl phthalate and mono(2-ethyl-...Continue Reading

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Citations

Jan 29, 2021·International Journal of Environmental Research and Public Health·Andrea TinelliRadmila Sparić
Feb 22, 2021·Women's Health Issues : Official Publication of the Jacobs Institute of Women's Health·Brianna N VanNoyAmi R Zota
Oct 13, 2020·Current Opinion in Endocrinology, Diabetes, and Obesity·Maria Victoria BarianiAmi R Zota
Jul 3, 2021·Journal of Personalized Medicine·Margherita FerranteGea Oliveri Conti

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Methods Mentioned

BETA
hysterectomy
Assay

Software Mentioned

R
miRbase
Ingenuity Pathway Analysis ( IPA )
Target Scan
QuantStudio
miRecords
Thermo Fisher Cloud Relative Quantification

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