PMID: 2117667Aug 5, 1990Paper

Phycobiliprotein methylation. Effect of the gamma-N-methylasparagine residue on energy transfer in phycocyanin and the phycobilisome

Journal of Molecular Biology
R V Swanson, A N Glazer

Abstract

The phycobiliproteins contain a conserved unique modified residue, gamma-N-methylasparagine at beta-72. This study examines the consequences of this methylation for the structure and function of phycocyanin and of phycobilisomes. An assay for the protein asparagine methylase activity was developed using [methyl-3H]S-adenosylmethionine and apophycocyanin purified from Escherichia coli containing the genes for the alpha and beta subunits of phycocyanin from Synechococcus sp. PCC 7002 as substrates. This assay permitted the partial purification, from Synechococcus sp. PCC 6301, of the activity that methylates phycocyanin and allophycocyanin completely at residue beta-72. Using the methylase assay, two independent nitrosoguanidine-induced mutants of Synechococcus sp. PCC 7942 were isolated that do not exhibit detectable phycobiliprotein methylase activity. These mutants, designated pcm 1 and pcm 2, produce phycocyanin and allophycocyanin unmethylated at beta-72. The phycobiliproteins in these mutants are assembled into phycobilisomes and can be methylated in vitro by the partially purified methylase from Synechococcus sp. PCC 6301. The mutants produce phycobiliproteins in amounts comparable to those of wild-type and the mutant and ...Continue Reading

References

Oct 26, 1989·Biochimica Et Biophysica Acta·P MaxsonA N Glazer
Jan 1, 1988·Methods in Enzymology·R Rippka
Jan 1, 1988·Methods in Enzymology·J Houmard, N T de Marsac
Jan 1, 1985·Annual Review of Biophysics and Biophysical Chemistry·A N Glazer
Jan 1, 1970·Molecular & General Genetics : MGG·S V Shestakov, N T Khyen
Jul 1, 1983·Annales de microbiologie·A N GlazerR C Williams
Jan 1, 1986·Biophysical Journal·A N Glazer, J H Clark

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Citations

Feb 21, 2008·Molecular Microbiology·H Scheer, K-H Zhao
Apr 14, 2016·Photosynthesis Research·Vinay KumarDatta Madamwar
Feb 7, 2002·Proceedings of the National Academy of Sciences of the United States of America·Steven Clarke
Nov 1, 1995·Photosynthesis Research·A N Glazer, G J Wedemayer
Oct 23, 2018·Oncotarget·Avilala JanardhanZhigang Guo
Nov 2, 2014·Photosynthesis Research·Natalia BattchikovaEva-Mari Aro
Jul 16, 2019·Biochimica Et Biophysica Acta. Bioenergetics·Noam AdirDvir Harris
May 16, 2001·Biochemical and Biophysical Research Communications·A K PadyanaS Ramakumar
Nov 15, 1991·Archives of Biochemistry and Biophysics·A V Klotz, B M Higgins
Dec 1, 1993·Current Opinion in Cell Biology·S Clarke
Oct 31, 2021·Communications Biology·Takuo MinatoKi-Seok Yoon

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