Phycocyanin-Functionalized Selenium Nanoparticles Reverse Palmitic Acid-Induced Pancreatic β Cell Apoptosis by Enhancing Cellular Uptake and Blocking Reactive Oxygen Species (ROS)-Mediated Mitochondria Dysfunction

Journal of Agricultural and Food Chemistry
Chang LiuXiaoling Li

Abstract

Accumulation of palmitic acid (PA) in human bodies could cause damage to pancreatic β cells and lead to chronic diseases by generation of reactive oxygen species (ROS). Therefore, it is of great significance to search for nutrition-available agents with antioxidant activity to protect pancreatic islet cells against PA-induced damage. Phycocyanin (PC) and selenium (Se) have been reported to have excellent antioxidant activity. In this study, PC-functionalized selenium nanoparticles (PC-SeNPs) were synthesized to investigate the in vitro protective effects on INS-1E rat insulinoma β cells against PA-induced cell death. A potent protective effect was achieved by regulation of particle size and PC content. Among three PC-SeNPs (165, 235, and 371 nm), PC-SeNPs-235 nm showed the highest cellular uptake and the best protective activities. For cell cycle analysis, PC-SeNPs showed a better protective effect on PA-induced INS-1E cell apoptosis than PC or SeNPs, and PC-SeNPs-235 nm exhibited the best effect. Further mechanistic studies demonstrated that PA induced overproduction of intracellular ROS, mitochondria fragmentation, activation of caspase-3, -8, and -9, and cleavage of PARP. However, pretreatment of the cells with PC-SeNPs effe...Continue Reading

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Citations

May 3, 2019·Toxicology Mechanisms and Methods·Xiaotian ZhangChanghao Liang
Oct 20, 2018·Reproductive Biology and Endocrinology : RB&E·Xie GeBing Yao
Oct 12, 2018·Nutrients·Diana Constantinescu-AruxandeiFlorin Oancea
Dec 2, 2019·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Yin ZhangWeiping Xie
Dec 11, 2020·Diabetes, Metabolic Syndrome and Obesity : Targets and Therapy·Xiao-Long LiaoWei-Xin Guo
Nov 27, 2018·Journal of Agricultural and Food Chemistry·Qingyun TanTianfeng Chen

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