Phylogenetic analysis and expression patterns of p16 and p19 in Paracentrotus lividus embryos.

Development Genes and Evolution
Caterina CostaV Matranga

Abstract

P16 and P19 are two small acidic proteins involved in the formation of the biomineralized skeleton of sea urchin embryos and adults. Here, we describe the cloning and the embryonic temporal and spatial expression profiles of p16 and p19 mRNAs, identified for the first time in Paracentrotus lividus. Phylogenetic analysis showed a high degree of similarity of the deduced Pl-P16 and Pl-P19 sequences with the Lytechinus variegatus and Strongylocentrotus purpuratus orthologs. While only a reduced similarity with other phyla, including mammals, was detected, their implication in biomineralized tissues calls for their conservation in evolution. By comparative quantitative PCR and in situ hybridization, we found that Pl-p16 and Pl-p19 expression was restricted to skeletogenic cells throughout embryogenesis, with transcript levels peaking at the late gastrula stage. Dissimilar Pl-p16 and Pl-p19 spatial expression within the primary mesenchyme cell syncytium at the gastrula and pluteus stages suggests the occurrence of a different regulation of gene transcription.

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Citations

Apr 25, 2015·Developmental and Comparative Immunology·Roberta RussoVincenzo Arizza
Mar 18, 2016·Toxicological Sciences : an Official Journal of the Society of Toxicology·Stefano VarrellaMaria Costantini
Sep 10, 2013·Marine Environmental Research·Roberta RussoValeria Matranga
May 28, 2016·Marine Biotechnology·Konstantinos KarakostisValeria Matranga
Aug 27, 2016·Scientific Reports·Nadia RuoccoLuigia Santella
Apr 25, 2017·Development, Growth & Differentiation·Daniele P RomancinoAntonella Bongiovanni
Apr 25, 2017·Ecotoxicology and Environmental Safety·Nadia RuoccoMaria Costantini
Apr 3, 2019·International Journal of Immunogenetics·Marco ChiaramonteRoberta Russo
Jul 28, 2020·International Journal of Molecular Sciences·Christian GalassoGiovanna Romano
Nov 13, 2017·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Letizia AnelloMaria Di Bernardo

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