Phylogenetic analysis and in situ identification of bacteria community composition in an acidic Sphagnum peat bog

Applied and Environmental Microbiology
S N DedyshW Liesack

Abstract

The Bacteria community composition in an acidic Sphagnum peat bog (pH 3.9 to 4.5) was characterized by a combination of 16S rRNA gene clone library analysis, rRNA-targeted fluorescence in situ hybridization (FISH), and cultivation. Among 84 environmental 16S rRNA gene clones, a set of only 16 cloned sequences was closely related (>or=95% similarity) to taxonomically described organisms. Main groups of clones were affiliated with the Acidobacteria (24 clones), Alphaproteobacteria (20), Verrucomicrobia (13), Actinobacteria (8), Deltaproteobacteria (4), Chloroflexi (3), and Planctomycetes (3). The proportion of cells that hybridized with oligonucleotide probes specific for members of the domains Bacteria (EUB338-mix) and Archaea (ARCH915 and ARC344) accounted for only 12 to 22% of the total cell counts. Up to 24% of the EUB338-positive cells could be assigned by FISH to specific bacterial phyla. Alphaproteobacteria and Planctomycetes were the most numerous bacterial groups (up to 1.3x10(7) and 1.1x10(7) cells g-1 peat, respectively). In contrast to conventional plating techniques, a novel biofilm-mediated enrichment approach allowed us to isolate some representatives of predominant Bacteria groups, such as Acidobacteria and Planct...Continue Reading

References

Jun 1, 1995·Applied and Environmental Microbiology·N WardH Schlesner
Mar 21, 1998·Applied and Environmental Microbiology·S N DedyshJ M Tiedje
Oct 6, 1998·Applied and Environmental Microbiology·M F Polz, C M Cavanaugh
Nov 11, 1999·International Journal of Systematic Bacteriology·J D CoatesD R Lovley
Jan 5, 2002·International Journal of Systematic and Evolutionary Microbiology·K J ChinP H Janssen
Jan 5, 2002·Applied and Environmental Microbiology·Jenny WangJohn A Fuerst
Feb 12, 2002·International Journal of Systematic and Evolutionary Microbiology·Svetlana N DedyshJames M Tiedje
Mar 2, 2002·Applied and Environmental Microbiology·Samantha A MorrisJ Colin Murrell
Jan 7, 2003·Applied and Environmental Microbiology·Marcus A HornHarold L Drake
Apr 25, 2003·International Journal of Systematic and Evolutionary Microbiology·Paolo MonciardiniStefano Donadio
Feb 27, 2004·Nucleic Acids Research·Wolfgang LudwigKarl-Heinz Schleifer
Apr 10, 2004·Bioinformatics·Thomas HuberPhilip Hugenholtz
Aug 6, 2004·Applied and Environmental Microbiology·Bradley S StevensonJohn A Breznak
Aug 27, 2005·Nature·Ashna A RaghoebarsingMarc Strous
May 1, 1991·Ecological Applications : a Publication of the Ecological Society of America·Eville Gorham

❮ Previous
Next ❯

Citations

Jan 31, 2012·Archives of Microbiology·Megan L Kempher, Michael T Madigan
May 12, 2012·Archives of Microbiology·Olga Maria LageJosé A M Catita
Oct 1, 2010·Indian Journal of Microbiology·M Rohini Kumar, V S Saravanan
Mar 1, 2008·The ISME Journal·Timofei A PankratovSvetlana N Dedysh
Nov 28, 2012·The ISME Journal·Chakkiath Paul AntonyYogesh S Shouche
Nov 14, 2008·Proceedings of the National Academy of Sciences of the United States of America·Wyatt H HartmanGregory L Bruland
Apr 2, 2008·Applied and Environmental Microbiology·Alexandra HambergerHarold L Drake
Apr 12, 2011·Applied and Environmental Microbiology·Sindy HungerHarold L Drake
Apr 26, 2011·Applied and Environmental Microbiology·Jaap S Sinninghe DamstéSvetlana N Dedysh
Nov 26, 2010·Applied and Environmental Microbiology·Stephanie A EichorstThomas M Schmidt
Aug 14, 2012·Applied and Environmental Microbiology·Astrid NaetherMichael W Friedrich
Feb 5, 2008·Applied and Environmental Microbiology·Sascha F PercentSandra A Nierzwicki-Bauer
Feb 13, 2007·Applied and Environmental Microbiology·Stephanie A EichorstThomas M Schmidt
Feb 10, 2009·Applied and Environmental Microbiology·Naomi L WardCheryl R Kuske
Mar 5, 2011·Microbiology and Molecular Biology Reviews : MMBR·Ryan J NewtonStefan Bertilsson
Feb 7, 2007·Doklady Biological Sciences : Proceedings of the Academy of Sciences of the USSR, Biological Sciences Sections·T A PankratovG A Zavarzin
Apr 16, 2011·Canadian Journal of Microbiology·Roland C WilhelmLyle G Whyte
Jun 28, 2007·PLoS Biology·Chana PalmerPatrick O Brown
Feb 6, 2008·PLoS Pathogens·Philip McKennaFrederic D Bushman
Feb 10, 2011·Standards in Genomic Sciences·Rüdiger PukallPhilip Hugenholtz
Jun 4, 2014·Research in Microbiology·Rafael Mazer EttoMaria Berenice Reynaud Steffens
May 15, 2013·Izvestiia Akademii nauk. Seriia biologicheskaia·E G LukachevaN A Manucharova
Feb 19, 2009·Environmental Microbiology·Pia K WüstHarold L Drake
Oct 1, 2009·Environmental Microbiology Reports·Harold L DrakePia K Wüst
May 14, 2011·Environmental Microbiology·Timofey A PankratovWerner Liesack
Mar 1, 2015·International Journal of Systematic and Evolutionary Microbiology·Irina S KulichevskayaSvetlana N Dedysh
Aug 23, 2011·International Journal of Systematic and Evolutionary Microbiology·Irina S KulichevskayaSvetlana N Dedysh

❮ Previous
Next ❯

Related Concepts

Related Feeds

Archaeogenetics

Recent advances in genomic sequencing has led to the discovery of new strains of Archaea and shed light on their evolutionary history. Discover the latest research on Archaeogenetics here.

Biofilm & Infectious Disease

Biofilm formation is a key virulence factor for a wide range of microorganisms that cause chronic infections.Here is the latest research on biofilm and infectious diseases.