PMID: 11900959Mar 20, 2002Paper

Phylogenetic analysis and PCR detection of Clostridium chauvoei, Clostridium haemolyticum, Clostridium novyi types A and B, and Clostridium septicum based on the flagellin gene

Veterinary Microbiology
Yoshimasa SasakiYutaka Tamura

Abstract

The flagellin genes (fliC) of Clostridium chauvoei, Clostridium haemolyticum, Clostridium novyi types A and B, and Clostridium septicum were analysed by PCR amplification and DNA sequencing. The five Clostridium species have at least two copies of the flagellin gene (fliC) arranged in tandem on the chromosome. The deduced N- and C-terminal aminoacid sequences of the flagellin proteins (FliCs) of these clostridia are well conserved but their central region aminoacid sequences are not. Phylogenic analysis based on the N-terminal aminoacid sequence of the FliC protein revealed that these clostridia, which belong to Clostridium 16S rDNA phylogenic cluster I (), are more closely related to Bacillus subtilis than to Clostridium difficile, which belongs to the cluster XI. Moreover, a multiplex polymerase reaction (PCR) system based on the fliC sequence was developed to rapidly identify C. chauvoei, C. haemolyticum, C. novyi types A and B, and C. septicum. PCR of each Clostridium amplified a species-specific band. The multiplex PCR system may be useful for rapid identification of pathogenic clostridia.

References

Aug 1, 1991·Journal of Bacteriology·P GuerryT J Trust
Dec 20, 1985·Journal of Molecular Biology·L N Wei, T M Joys
Oct 1, 1994·International Journal of Systematic Bacteriology·M D CollinsJ A Farrow
Sep 23, 2000·Veterinary Microbiology·A KojimaY Tamura
Jun 20, 2001·International Journal of Systematic and Evolutionary Microbiology·Y SasakiY Tamura

❮ Previous
Next ❯

Citations

Mar 14, 2007·Applied and Environmental Microbiology·Catherine J PaulSusan M Logan
Feb 8, 2008·Journal of Clinical Microbiology·Noriyuki NaganoYoshichika Arakawa
Feb 15, 2011·Veterinary Research·Edy M VileiJoachim Frey
Oct 16, 2010·The Journal of Veterinary Medical Science·Yasunori ShinozukaMitsuhiro Takagi
Jun 20, 2012·Anaerobe·V EeckhautF Van Immerseel
Aug 2, 2013·Journal of Medical Microbiology·Barbara SeiseKarina Weber
May 17, 2006·Anaerobe·Yuli Song
Feb 23, 2020·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Carlos A Oliveira JuniorFrancisco A Uzal
Nov 19, 2019·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Mauricio A Navarro, Francisco A Uzal
Jul 27, 2007·Scandinavian Journal of Infectious Diseases·Nahed M Abdel-HaqBasim I Asmar
Oct 28, 2019·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Rosangela Estel ZiechAgueda Castagna de Vargas
Dec 12, 2017·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Akinyi C NyaokeFrancisco A Uzal
Jun 11, 2017·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Camila C AbreuFrancisco A Uzal
May 8, 2020·BMC Veterinary Research·Chang-Gi JeongWon-Il Kim
Aug 9, 2016·The Journal of Veterinary Medical Science·Mitsuhiro TakagiOsamu Yamato
Dec 20, 2018·BMC Veterinary Research·Abdullah GaziogluBurhan Çetinkaya
Jul 28, 2021·Vaccine·Mariliana Luiza Ferreira AlvesFabricio Rochedo Conceição

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.