Phylogenetic analysis of an epidemic outbreak of acute hepatitis C in HIV-infected patients by ultra-deep pyrosequencing

Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology
Noelia Caro-PérezSofía Pérez-Del-Pulgar

Abstract

The incidence of acute hepatitis C (AHC) among HIV-infected men who have sex with men (MSM) has increased significantly in the last 10 years. Several studies point to a social and sexual network of HIV-positive MSM that extends internationally. The aim of our study was to investigate the dynamics of HCV transmission in an outbreak of AHC in HIV-infected MSM in Barcelona by ultra-deep pyrosequencing. Between 2008 and 2013, 113 cases of AHC in HIV-infected MSM were diagnosed in the Infectious Diseases Unit, Hospital Clínic, Barcelona. Massive sequencing was performed using the Roche 454 GS Junior platform. To define possible transmission networks, maximum likelihood phylogenetic trees were constructed, and levels of genetic diversity within and among patients were compared. Among the 70 cases analyzed, we have identified 16 potential clusters of transmission: 8 for genotype 1a (23 cases involved), 1 for genotype 1b (3 cases) and 7 for genotype 4d (27 cases). Although the initial phylogenetic reconstruction suggested a local transmission cluster of HCV gt4d, our approach based on low genetic differentiation did not corroborate it. Indeed, gt4d strains formed 4 independent groups related to patients from other countries. Frequent c...Continue Reading

Citations

Feb 28, 2019·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Christophe RamièreUNKNOWN Lyon Acute Hepatitis Study Group
Jun 7, 2019·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Thuy NguyenEve Todesco
Jul 23, 2019·Journal of Viral Hepatitis·Anna MaisaUNKNOWN Outbreak Control Team
Aug 7, 2020·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Jürgen Kurt Rockstroh
Jul 4, 2018·Future Virology·Juan Á Patiño-Galindo, Fernando González-Candelas
Oct 12, 2018·Viruses·Pierre PradatEric Trépo
Dec 29, 2020·HIV Medicine·A Gonzalez-SernaUNKNOWN HEPAVIR study group
Mar 1, 2020·Enfermedades infecciosas y microbiología clínica·Iñaki ComasFernando González-Candelas
Oct 1, 2018·Gastroenterology·Anna L McNaughtonPhilippa C Matthews
Feb 19, 2020·Journal of Microbiology, Immunology, and Infection = Wei Mian Yu Gan Ran Za Zhi·Wei-You LiYi-Ming Arthur Chen

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