Phylogenetic analysis of Hungarian goose parvovirus isolates and vaccine strains

Avian Pathology : Journal of the W.V.P.A
Tímea Tatár-KisVilmos Palya

Abstract

Polymerase chain reaction and sequencing were used to analyse goose parvovirus field isolates and vaccine strains. Two fragments of the genome were amplified. Fragment "A" represents a region of VP3 gene, while fragment "B" represents a region upstream of the VP3 gene, encompassing part of the VP1 gene. In the region of fragment "A" the deduced amino acid sequence of the strains was identical, therefore differentiation among strains could be done only at the nucleotide level, which resulted in the formation of three groups: Hungarian, West-European and Asian strains. In the region of fragment "B", separation of groups could be done by both nucleotide and deduced amino acid sequence level. The nucleotide sequences resulted in the same groups as for fragment "A" but with a different clustering pattern among the Hungarian strains. Within the "Hungarian" group most of the recent field isolates fell into one cluster, very closely related or identical to each other, indicating a very slow evolutionary change. The attenuated strains and field isolates from 1979/80 formed a separate cluster. When vaccine strains and field isolates were compared, two specific amino acid differences were found that can be considered as possible markers f...Continue Reading

Citations

Mar 16, 2006·Avian Pathology : Journal of the W.V.P.A·Dave Cavanagh
Mar 27, 2009·Avian Pathology : Journal of the W.V.P.A·Vilmos PalyaTamás Mató
Dec 3, 2013·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Ming Li, Tian-fei Yu
Feb 19, 2016·Transboundary and Emerging Diseases·H ChenY Diao
Jun 19, 2015·Archives of Virology·Shao WangJingxiang Wang
Apr 20, 2016·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Shao WangYou-Quang Cheng
Dec 4, 2014·Veterinary Microbiology·Yumin ZhuYuanshu Zhang
Jun 17, 2016·Avian Diseases·Awad A ShehataThomas W Vahlenkamp
Aug 16, 2016·Journal of Virological Methods·Xiaoyu NiuYouxiang Diao
Sep 25, 2018·Avian Pathology : Journal of the W.V.P.A·Sunil S KapgateSukhadeo B Barbuddhe
Sep 9, 2019·Archives of Virology·Meiling JinDabing Zhang
Nov 25, 2020·Tropical Animal Health and Production·Özlem Kardoğanİnci Başak Müştak
Apr 9, 2005·The Veterinary Record·Dick GoughTamas Mato
Dec 19, 2020·Animals : an Open Access Journal From MDPI·Anna Karolina MatczukAlina Wieliczko

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Datasets Mentioned

BETA
AY496890
AY496903
AY496884
AY496885
AY496898
AY496887
U22967

Methods Mentioned

BETA
PCR

Software Mentioned

DNAmlk
TREECON
BioEdit

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