Phylogenetic analysis of intestinal bacteria in the Chinese mitten crab (Eriocheir sinensis)

Journal of Applied Microbiology
K LiY Zhang

Abstract

To identify the dominant intestinal bacteria in the Chinese mitten crab, and to investigate the differences in the intestinal bacteria between pond-raised and wild crabs. The diversity of intestinal bacteria in the Chinese mitten crabs was investigated by denaturing gradient gel electrophoresis (DGGE) fingerprinting, 16S rRNA gene clone library analysis and real-time quantitative PCR. The principal component analysis of DGGE profiles indicated that substantial intersubject variations existed in intestinal bacteria in pond-raised crab. The sequencing of 16S rRNA genes revealed that 90-95% of the phylotypes in the clone libraries were affiliated with Proteobacteria and Bacteroidetes. Some genera were identified as unique in wild crabs and in pond-raised crabs, whereas Bacteroidetes was found to be common in all sampled crab groups. Real-time quantitative PCR indicated that the abundance of Bacteroides and the total bacterial load were approximately four-to-10 times higher in pond-raised crabs than in wild crabs. A significant portion of the phylotypes shared low similarity with previously sequenced organisms, indicating that the bacteria in the gut of Chinese mitten crabs are yet to be described. The intestinal bacteria of pond-r...Continue Reading

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Citations

Apr 9, 2011·Standards in Genomic Sciences·Birte AbtNikos C Kyrpides
Oct 9, 2012·Systematic and Applied Microbiology·Alexandra MezitiKonstantinos Ar Kormas
Feb 14, 2012·Comptes rendus biologies·Laure CorbariPhilippe Compère
Mar 12, 2008·Environmental Microbiology·Massimo MarzoratiWilly Verstraete
Nov 3, 2010·Journal of Separation Science·Scott S SegroAbdul Malik
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Jul 26, 2018·Frontiers in Physiology·Yi-Feng LiJin-Long Yang

Related Concepts

Bacteroides
Uca
DNA, Bacterial
SDS-PAGE
Food Microbiology
Intestines
Phylogeny
RNA, Bacterial
RNA, Ribosomal, 16S
Variation (Genetics)

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