PMID: 11911592Mar 26, 2002Paper

Phylogenetic analysis of the 5'-untranslated region of german BVDV type II isolates

Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health
M BeerO R Kaaden

Abstract

On the basis of genetic differences, bovine viral diarrhoea viruses (BVDV) are subclassified into two distinct genotypes, BVDV type I and BVDV type II. We selected German BVDV type II isolates using the BVDV type I-specific monoclonal antibody WB160 and flow cytometric analysis for further characterization. For molecular characterization, a 288-bp fragment of the 5'-untranslated region (5'-UTR) of the selected isolates was investigated by reverse transcription-polymerase chain reaction (RT-PCR) and sequencing. Sequence comparisons of the partial 5'-UTR sequences and their phylogenetic analyses demonstrated that the 18 German BVDV type II isolates all belong to either subtype IIa (10 isolates) or subtype IIc (eight isolates). Nevertheless, the German BVDV type II isolates were genetically different (89.9-94.3% sequence identity) from the standard BVDV type II strain 890 from North America, which was recently classified as BVDV type IIa. Furthermore, a clear subdivision of the German BVDV type II isolates into two distinct subtypes (BVDV IIa Germany and BVDV IIc Germany) is shown. Viruses of both subgroups differed in the analysed 5'-UTR fragment from each other (91.6-95.2% sequence identity), but were highly conserved within the...Continue Reading

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Citations

Mar 13, 2010·Tropical Animal Health and Production·Tuba Cigdem OguzogluIbrahim Burgu
Feb 28, 2003·Virus Research·A HurtadoR A Juste
Aug 28, 2007·Journal of Virological Methods·Massimo Giangaspero, Ryô Harasawa
Sep 20, 2005·Preventive Veterinary Medicine·C LetellierP Kerkhofs
Oct 23, 2016·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Francesco CeruttiSimone Peletto
Oct 9, 2016·BMC Veterinary Research·Frederike ReichmannCarola M Sauter-Louis
Jul 13, 2016·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Aspen M WorkmanCarol G Chitko-McKown

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