Phylogenetic and metabolic diversity of bacteria associated with cystic fibrosis.

The ISME Journal
Adam M GussColleen M Cavanaugh

Abstract

In patients afflicted with cystic fibrosis (CF), morbidity and mortality are primarily associated with the adverse consequences of chronic microbial bronchial infections, which are thought to be caused by a few opportunistic pathogens. However, recent evidence suggests the presence of other microorganisms, which may significantly affect the course and outcome of the infection. Using a combination of 16S rRNA gene clone libraries, bacterial culturing and pyrosequencing of barcoded 16S rRNA amplicons, the microbial communities present in CF patient sputum samples were examined. In addition to previously recognized CF pathogens such as Pseudomonas aeruginosa and Staphylococcus aureus, >60 phylogenetically diverse bacterial genera that are not typically associated with CF pathogenesis were also detected. A surprisingly large number of fermenting facultative and obligate anaerobes from multiple bacterial phyla was present in each sample. Many of the bacteria and sequences found were normal residents of the oropharyngeal microflora and with many containing opportunistic pathogens. Our data suggest that these undersampled organisms within the CF lung are part of a much more complex microbial ecosystem than is normally presumed. Charac...Continue Reading

References

Mar 1, 1971·Journal of Bacteriology·S K Ng, I R Hamilton
Apr 1, 1995·American Journal of Respiratory and Critical Care Medicine·T Z KhanD W Riches
Nov 14, 1997·American Journal of Respiratory and Critical Care Medicine·D De BackerJ L Vincent
Jun 1, 1997·Periodontology 2000·R C Page, K S Kornman
Sep 12, 1998·International Journal of Systematic Bacteriology·B J PasterR L Ericson
Oct 6, 1998·Applied and Environmental Microbiology·M F Polz, C M Cavanaugh
Feb 2, 1999·The Journal of Clinical Investigation·R BalsJ M Wilson
Aug 4, 1999·Molecular Medicine Today·B Tümmler, C Kiewitz
May 24, 2001·Journal of Bacteriology·B J PasterF E Dewhirst
Feb 6, 2002·The Journal of Clinical Investigation·Dieter WorlitzschGerd Döring
Apr 5, 2002·Clinical Microbiology Reviews·Jeffrey B LyczakGerald B Pier
Jun 22, 2002·Science·Deborah A Hogan, Roberto Kolter
Oct 14, 2003·American Journal of Respiratory and Critical Care Medicine·Ronald L GibsonBonnie W Ramsey
Mar 16, 2004·Proceedings of the National Academy of Sciences of the United States of America·Zhiheng PeiMartin J Blaser
Sep 11, 2004·Journal of Tropical Pediatrics·A Abdul WahabS El-Shafie
Jan 5, 2005·Journal of Bacteriology·Lauren M MashburnMarvin Whiteley
Jan 18, 2005·Lancet·Kim A BrogdenChristopher E Taylor
Apr 16, 2005·Science·Paul B EckburgDavid A Relman
Sep 16, 2005·Nature·Lauren M Mashburn, Marvin Whiteley
Nov 8, 2005·Journal of Clinical Microbiology·Jørn A AasFloyd E Dewhirst
Jan 13, 2006·Proceedings of the National Academy of Sciences of the United States of America·Elisabeth M BikDavid A Relman
Aug 2, 2006·Proceedings of the National Academy of Sciences of the United States of America·Mitchell L SoginGerhard J Herndl
Aug 26, 2006·Periodontology 2000·Bruce J PasterFloyd E Dewhirst
Nov 23, 2006·Proceedings of the National Academy of Sciences of the United States of America·Hirotoshi MatsuiRichard C Boucher
Mar 24, 2007·Microbiology·Freya Harrison
Apr 3, 2007·PLoS Computational Biology·Eric P Nawrocki, Sean R Eddy
Apr 10, 2007·Current Opinion in Pharmacology·Marisa I Gómez, Alice Prince
Jun 26, 2007·Applied and Environmental Microbiology·Qiong WangJames R Cole
Sep 18, 2007·Journal of Bacteriology·Kelli L PalmerMarvin Whiteley
Dec 14, 2007·Proceedings of the National Academy of Sciences of the United States of America·J Kirk HarrisNorman R Pace
Feb 12, 2008·American Journal of Respiratory and Critical Care Medicine·Michael M TunneyJ Stuart Elborn
Apr 3, 2008·PloS One·Susannah G TringeYijun Ruan
Jun 11, 2008·Cell Host & Microbe·Thierry Pédron, Philippe Sansonetti
Sep 25, 2008·Proceedings of the National Academy of Sciences of the United States of America·Christopher D SibleyMichael G Surette
Oct 15, 2008·Systematic Biology·Alexandros StamatakisJacques Rougemont

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Citations

Jul 29, 2011·The ISME Journal·Dana WillnerDouglas Conrad
Aug 9, 2012·Proceedings of the National Academy of Sciences of the United States of America·Amanda F GoddardPradeep K Singh
Mar 28, 2012·Proceedings of the National Academy of Sciences of the United States of America·Jiangchao ZhaoJohn J LiPuma
Sep 29, 2012·Science Translational Medicine·Paul C BlaineyStephen R Quake
Jan 15, 2011·Clinical Microbiology Reviews·Alan R HauserSusanna A McColley
Aug 24, 2012·Journal of Clinical Microbiology·Raúl Cabrera-RubioAlex Mira
Sep 28, 2012·Journal of Clinical Microbiology·Francisca Hernández-HernándezLaurence Delhaes
Feb 10, 2012·Thorax·Meilan K HanFernando J Martinez
Jan 19, 2013·American Journal of Respiratory and Critical Care Medicine·Dana L WillnerDaniel C Chambers
Jan 26, 2013·American Journal of Respiratory and Critical Care Medicine·Michael M TunneyJ Stuart Elborn
Aug 7, 2012·American Journal of Respiratory Cell and Molecular Biology·Ryan C HunterDianne K Newman
Feb 1, 2013·PloS One·Man Kit CheungStephen Kwok Wing Tsui
Aug 3, 2013·Biomicrofluidics·M SkolimowskiJ Emnéus
Mar 7, 2014·BMC Medical Genetics·Fernando Augusto de Lima MarsonJose Dirceu Ribeiro
Jan 28, 2014·Annals of the American Thoracic Society·Leopoldo N SegalMichael D Weiden
Jan 31, 2013·Future Medicinal Chemistry·Matthew Hurley, Alan Smyth
Jun 28, 2013·Annals of the American Thoracic Society·Lisa A CarmodyJohn J LiPuma
May 6, 2014·Trends in Molecular Medicine·Matthew HartfieldSamuel Alizon
Jun 24, 2014·Genomics, Proteomics & Bioinformatics·Zhancheng GaoLufeng Ren
Mar 22, 2014·Critical Reviews in Microbiology·Susana P LopesMaria O Pereira
Feb 8, 2014·Expert Review of Anti-infective Therapy·Poonam SharmaJean-Marc Rolain
Apr 8, 2014·Expert Review of Vaccines·Richard B KennedyGregory A Poland
Dec 8, 2015·Applied Microbiology and Biotechnology·Andreia P MagalhãesSusana P Lopes
Sep 20, 2012·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Stig Bengmark
Dec 15, 2015·Scientific Reports·Christos GiatsisMarc Verdegem
Dec 2, 2015·The ISME Journal·Robert A QuinnForest L Rohwer
Aug 22, 2013·Microbial Ecology·Shu AnMichael S DuBow
Apr 20, 2016·Frontiers in Microbiology·Sarah K HiltonKeith A Crandall
Sep 4, 2015·Pediatric Pulmonology·Lindsay J CaverlyJohn J LiPuma

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