Apr 25, 2020

Diversity and gene expression patterns of functional groups in sidestream and mainstream wastewater partial-nitritation anammox biofilms

BioRxiv : the Preprint Server for Biology
Carolina SuarezF. Persson

Abstract

Partial nitritation-anammox (PNA) is today used for nitrogen removal from highly concentrated wastewater after anaerobic sludge digestion (sidestream). However, implementation of PNA for treatment of municipal wastewater (mainstream), with its lower ammonium concentration and lower temperature is challenging, which might be due to differences in microbial community composition and/or activity. To investigate this, we compared side-by-side sidestream and mainstream PNA biofilms using amplicon sequencing of 16S rDNA and rRNA, hzsB DNA and mRNA, and the genes nxrB, and amoA. The two communities were different to each other with relatively more heterotrophic denitrifying bacteria and less anammox bacteria in the mainstream. With hzsB and nxrB we found microdiversity among Brocadia and Nitrospira, and turnover (taxa replacement) between sidestream and mainstream. However, in both environments Brocadia sapporoensis represented most of the hzsB DNA and mRNA reads, despite the different environmental conditions and nitrogen removal rates. All of those populations present in both sidestream and mainstream had no differences in their 16S rRNA:rDNA ratios, supporting recent findings that rRNA:rDNA ratios are poor indicators of bacterial a...Continue Reading

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Mentioned in this Paper

Study
Bacterial cell
Protein Binding
Genome
Transcription, Genetic
Body Image
N-(4-aminophenethyl)spiroperidol
Macromolecular Alteration
Phylogenetic Analysis
Integration Host Factors

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