Phylogenetic characterization of the epibiotic bacteria associated with the hydrothermal vent polychaete Alvinella pompejana.

Applied and Environmental Microbiology
A HaddadS C Cary

Abstract

Alvinella pompejana is a polychaetous annelid that inhabits active deep-sea hydrothermal vent sites along the East Pacific Rise, where it colonizes the walls of actively venting high-temperature chimneys. An abundant, morphologically diverse epibiotic microflora is associated with the worm's dorsal integument, with a highly integrated filamentous morphotype clearly dominating the microbial biomass. It has been suggested that this bacterial population participates in either the nutrition of the worm or in detoxification of the worm's immediate environment. The primary goal of this study was to phylogenetically characterize selected epibionts through the analysis of 16S rRNA gene sequences. Nucleic acids were extracted from bacteria collected from the dorsal surface of A. pompejana. 16S rRNA genes were amplified with universal bacterial primers by the PCR. These genes were subsequently cloned, and the resulting clone library was screened by restriction fragment length polymorphism analysis to identify distinct clone types. The restriction fragment length polymorphism analysis identified 32 different clone families in the library. Four of these families were clearly dominant, representing more than 65% of the library. Representati...Continue Reading

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Citations

Nov 27, 2002·Applied and Environmental Microbiology·Mircea PodarMitchell L Sogin
Sep 6, 2003·Applied and Environmental Microbiology·Annette Summers EngelMichael Wagner
Jan 27, 2004·International Journal of Systematic and Evolutionary Microbiology·M L MiroshnichenkoE Stackebrandt
Oct 7, 2004·Applied and Environmental Microbiology·Barbara J Campbell, S Craig Cary
May 3, 2006·Nature Reviews. Microbiology·Barbara J CampbellKen Takai
Apr 1, 2003·FEMS Microbiology Ecology·Julie A HuberJohn A Baross
Aug 2, 2003·International Journal of Systematic and Evolutionary Microbiology·Nadège BrisbarreBernard Ollivier
Apr 3, 2001·Applied and Environmental Microbiology·V M MadridA Y Chistoserdov
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Dec 18, 2007·Applied and Environmental Microbiology·Charles K LeeRoy M Daniel
Nov 7, 2008·Proceedings of the National Academy of Sciences of the United States of America·Joseph J GrzymskiStephen C Cary
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