Phylogenetic Clades 6 and 8 of Enterohemorrhagic Escherichia coli O157:H7 With Particular stx Subtypes are More Frequently Found in Isolates From Hemolytic Uremic Syndrome Patients Than From Asymptomatic Carriers

Open Forum Infectious Diseases
Sunao IyodaJun Yatsuyanagi

Abstract

Enterohemorrhagic Escherichia coli (EHEC) O157:H7 infection causes severe diseases such as bloody diarrhea and hemolytic uremic syndrome (HUS). Although EHEC O157:H7 strains have exhibited high genetic variability, their abilities to cause human diseases have not been fully examined. Clade typing and stx subtyping of EHEC O157:H7 strains, which were isolated in Japan during 1999-2011 from 269 HUS patients and 387 asymptomatic carriers (ACs) and showed distinct pulsed-field gel electrophoresis patterns, were performed to determine relationships between specific lineages and clinical presentation. Clades 6 and 8 strains were more frequently found among the isolates from HUS cases than those from ACs (P = .00062 for clade 6, P < .0001 for clade 8). All clade 6 strains isolated from HUS patients harbored stx2a and/or stx2c, whereas all clade 8 strains harbored either stx2a or stx2a/stx2c. However, clade 7 strains were predominantly found among the AC isolates but less frequently found among the HUS isolates, suggesting a significant association between clade 7 and AC (P < .0001). Logistic regression analysis revealed that 0-9 year old age is a significant predictor of the association between clade 8 and HUS. We also found an intact...Continue Reading

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Citations

Oct 23, 2016·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Lucía GalliGerardo Aníbal Leotta
Aug 3, 2018·Japanese Journal of Infectious Diseases·Mihaela OpreaCodruţa-Romaniţa Usein
Feb 22, 2017·Journal of Food Protection·Umid M SharapovUNKNOWN OUTBREAK INVESTIGATION TEAM
Apr 27, 2017·Frontiers in Cellular and Infection Microbiology·Pragathi B ShridharJianfa Bai
May 28, 2017·Applied and Environmental Microbiology·Jay N WorleyEdward R Atwill
Aug 23, 2020·Nature Reviews. Microbiology·Erick DenamurDavid Gordon
Jun 26, 2020·Frontiers in Cellular and Infection Microbiology·Lin TengKwangcheol Casey Jeong
May 21, 2019·Microbial Genomics·Danielle J IngleDeborah A Williamson
Dec 17, 2020·Frontiers in Cellular and Infection Microbiology·Moo-Seung LeeVernon L Tesh
Nov 10, 2021·Microbial Genomics·Stephen F FitzgeraldDavid L Gally

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Methods Mentioned

BETA
electrophoresis
PCR

Software Mentioned

EpiInfo
BioNumerics

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