Phylogenetic classification of serotype III group B streptococci on the basis of hylB gene analysis and DNA sequences specific to restriction digest pattern type III-3

The Journal of Infectious Diseases
John F BohnsackElisabeth E Adderson

Abstract

Previous work divided serotype III group B streptococci (GBS) into 3 major phylogenetic lineages (III-1, III-2, and III-3) on the basis of bacterial DNA restriction digest patterns (RDPs). Most neonatal invasive disease was caused by III-3 strains, which implies that III-3 strains are more virulent than III-2 or III-1 strains. In the current studies, all RDP III-3 and III-1 strains expressed hyaluronate lysase activity; however, all III-2 strains lack hyaluronate lysase activity, because the gene that encodes hyaluronate lysase, hylB, is inactivated by IS1548. Subtractive hybridization was used to identify 9 short DNA sequences that are present in all the III-3 strains but not in any of the III-2 or III-1 strains. With 1 exception, these III-3-specific sequences were not detected in nonserotype III GBS. These data further validate the RDP-based subclassification of GBS and suggest that lineage-specific genes will be identified, which account for the differences in virulence among the lineages.

Citations

Apr 1, 2009·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·E van ElzakkerA van Belkum
Jan 18, 2005·Clinical Microbiology Reviews·Gunnar LindahlThomas Areschoug
Jun 7, 2003·Journal of Clinical Microbiology·Nicola JonesBrian G Spratt
Oct 22, 2004·Emerging Infectious Diseases·John F BohnsackMarcelo Gottschalk
Jan 1, 2008·Interdisciplinary Perspectives on Infectious Diseases·Lixin ZhangBetsy Foxman
Nov 11, 2008·Immunological Investigations·M A De FrancescoN Manca
May 17, 2002·Current Opinion in Infectious Diseases·Reinhard Berner

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