Phylogenetic diversity of bacterial symbionts of Solemya hosts based on comparative sequence analysis of 16S rRNA genes.

Applied and Environmental Microbiology
D M Krueger, C M Cavanaugh

Abstract

The bacterial endosymbionts of two species of the bivalve genus Solemya from the Pacific Ocean, Solemya terraeregina and Solemya pusilla, were characterized. Prokaryotic cells resembling gram-negative bacteria were observed in the gills of both host species by transmission electron microscopy. The ultrastructure of the symbiosis in both host species is remarkably similar to that of all previously described Solemya spp. By using sequence data from 16S rRNA, the identity and evolutionary origins of the S. terraeregina and S. pusilla symbionts were also determined. Direct sequencing of PCR-amplified products from host gill DNA with primers specific for Bacteria 16S rRNA genes gave a single, unambiguous sequence for each of the two symbiont species. In situ hybridization with symbiont-specific oligonucleotide probes confirmed that these gene sequences belong to the bacteria residing in the hosts gills. Phylogenetic analyses of the 16S rRNA gene sequences by both distance and parsimony methods identify the S. terraeregina and S. pusilla symbionts as members of the gamma subdivision of the Proteobacteria. In contrast to symbionts of other bivalve families, which appear to be monophyletic, the S. terraeregina and S. pusilla symbionts ...Continue Reading

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Citations

Jan 19, 1999·Annual Review of Microbiology·J M ShivelyW G Meijer
May 26, 1999·Critical Reviews in Microbiology·D CorsaroM Valassina
Aug 26, 1998·Proceedings of the National Academy of Sciences of the United States of America·A S PeekR C Vrijenhoek
Sep 26, 2001·Applied and Environmental Microbiology·J GulledgeC M Cavanaugh
Oct 10, 2006·Antonie van Leeuwenhoek·Frank J Stewart, Colleen M Cavanaugh
Jan 22, 2008·Applied and Environmental Microbiology·Ryan T JonesAndrew P Martin
Jun 17, 2010·FEMS Microbiology Ecology·Clara F RodriguesAndrew J Weightman
Apr 1, 2000·Applied and Environmental Microbiology·A R SipeS C Cary
Jul 28, 2009·Applied and Environmental Microbiology·Frank J StewartColleen M Cavanaugh
Jan 3, 2001·Applied and Environmental Microbiology·K RavenschlagR Amann
Mar 11, 2000·Molecular Biology and Evolution·A H van HoekJ H Hackstein
Jul 14, 2017·Proceedings. Biological Sciences·Brandon K B SeahHarald R Gruber-Vodicka

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