Phylogeny and characterization of three nifH-homologous genes from Paenibacillus azotofixans.

Applied and Environmental Microbiology
Quok-Cheong ChooNazalan Najimudin

Abstract

In this paper, we report the cloning and characterization of three Paenibacillus azotofixans DNA regions containing genes involved in nitrogen fixation. Sequencing analysis revealed the presence of nifB1H1D1K1 gene organization in the 4,607-bp SacI DNA fragment. This is the first report of linkage of a nifB open reading frame upstream of the structural nif genes. The second (nifB2H2) and third (nifH3) nif homologues are confined within the 6,350-bp HindIII and 2,840-bp EcoRI DNA fragments, respectively. Phylogenetic analysis demonstrated that NifH1 and NifH2 form a monophyletic group among cyanobacterial NifH proteins. NifH3, on the other hand, clusters among NifH proteins of the highly divergent methanogenic archaea.

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Citations

Sep 24, 2004·Journal of Biosciences·H S Jayasinghearachchi, Gamini Seneviratne
Nov 1, 2011·Journal of Bacteriology·Anelise BeneduziLuciane M P Passaglia
Jun 21, 2011·PloS One·Camila FernandezOsvaldo Ulloa
Feb 20, 2016·Genome Announcements·Mardani Abdul HalimNazalan Najimudin
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Jan 26, 2005·FEMS Microbiology Letters·Jigjiddorj Enkh-AmgalanTatsuji Seki

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