PMID: 11313131Apr 21, 2001Paper

Phylogeny and distribution of the soxB gene among thiosulfate-oxidizing bacteria

FEMS Microbiology Letters
R PetriJohannes F Imhoff

Abstract

A PCR protocol for the detection of sulfur-oxidizing bacteria based on soxB genes that are essential for thiosulfate oxidation by sulfur-oxidizing bacteria of various phylogenetic groups which use the 'Paracoccus sulfur oxidation' pathway was developed. Five degenerate primers were used to specifically amplify fragments of soxB genes from different sulfur-oxidizing bacteria previously shown to oxidize thiosulfate. The PCR yielded a soxB fragment of approximately 1000 bp from most of the bacteria. Amino acid and nucleotide sequences of soxB from reference strains as well as from new isolates and environmental DNA from a hydrothermal vent habitat in the North Fiji Basin were compared and used to infer relationships of soxB between sulfur-oxidizing bacteria belonging to various 16S rDNA-based phylogenetic groups. Major phylogenetic lines derived from 16S rDNA were confirmed by soxB phylogeny. Thiosulfate-oxidizing green sulfur bacteria formed a coherent group by their soxB sequences. Likewise, clearly separated branches demonstrated the distant relationship of representatives of alpha-, beta-, and gamma-Proteobacteria including representative species of the former genus Thiobacillus (now Halothiobacillus - gamma-Proteobacteria, Th...Continue Reading

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Citations

Dec 3, 2011·The ISME Journal·Joan M BernhardDavid J Beaudoin
Jun 19, 2009·The Journal of Biological Chemistry·Véronique SauvéBen C Berks
Sep 23, 2008·Applied and Environmental Microbiology·Nancy N PerreaultLyle G Whyte
May 21, 2013·Applied and Environmental Microbiology·Masrure AlamWriddhiman Ghosh
Jan 22, 2008·Applied and Environmental Microbiology·Debora Frigi Rodrigues, James M Tiedje
May 27, 2008·Applied and Environmental Microbiology·Marianne QuéméneurCatherine Joulian
Sep 6, 2003·Applied and Environmental Microbiology·Mostafa S ElshahedLee R Krumholz
Feb 10, 2004·Applied and Environmental Microbiology·Michinari SunamuraAkihiko Maruyama
Jun 26, 2012·BMC Microbiology·Vicente Gomez-AlvarezJorge W Santo Domingo
Nov 16, 2006·PLoS Biology·Kathleen M ScottGary T Zeruth
Jan 14, 2010·Applied Biochemistry and Biotechnology·Kishore Kumar KrishnaniS M Pillai
Feb 4, 2009·Current Microbiology·Shachindra K PandeySubrata K Das
Jan 8, 2011·Applied Microbiology and Biotechnology·Jian-Fei LuoYong Guo
Feb 26, 2011·FEMS Microbiology Ecology·Valentin CrépeauAnne Godfroy
Aug 17, 2018·Environmental Technology·Vinita Vishwakarma, Balakrishnan Anandkumar
Jan 21, 2015·Journal of Environmental Quality·Fatemeh Sanaei Moghadam, Martin Zimmer
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Sep 28, 2007·Canadian Journal of Microbiology·R AnandhamTongmin Sa
Oct 11, 2011·Biofouling·Jorge W Santo DomingoJames Weast

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