Phylogeny of not-yet-cultured spirochetes from termite guts.

Applied and Environmental Microbiology
B J PasterJ A Breznak

Abstract

Comparisons of 16S rDNA sequences were used to determine the phylogeny of not-yet-cultured spirochetes from hindguts of the African higher termite, Nasutitermes lujae (Wasmann). The 16S rRNA genes were amplified directly from spirochete-rich hindguts by using universal primers, and the amplified products were cloned into Escherichia coli. Clones were screened with a spirochete-specific DNA probe. Analysis of 1,410 base positions of the 16S rDNA insert from one spirochete clone, designated NL1, supported its assignment to the genus Treponema, with average interspecies similarities of ca. 85%. The sequence of NL1 was most closely related (ca. 87 to 88% similarity) to sequences of Spirochaeta stenostrepta and Spirochaeta caldaria and to a previously published sequence (ca. 87% similarity) of spirochetal clone MDS1 from the Australian lower termite, Mastotermes darwiniensis (Froggatt). On the basis of 16S rRNA sequence comparisons and individual base signatures, clones NL1 and MDS1 clearly represent two novel species of Treponema, although specific epithets have not yet been proposed. The gross morphology of NL1 was determined from in situ hybridization experiments with an NL1-specific, fluorescently labeled oligonucleotide probe. ...Continue Reading

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Citations

Jan 29, 1999·Science·J R LeadbetterJ A Breznak
Nov 24, 1999·FEMS Microbiology Letters·M OhkumaT Kudo
Jun 1, 2002·Applied and Environmental Microbiology·Janet E HillSean M Hemmingsen
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Jun 1, 2009·Indian Journal of Microbiology·R Rajagopal
Apr 7, 2005·Applied and Environmental Microbiology·Joseph R Graber, John A Breznak
Jan 5, 2006·Applied and Environmental Microbiology·Stefan DrögeHelmut König
Mar 26, 2003·Oral Microbiology and Immunology·F NakazawaT Ogawa
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May 3, 2008·International Journal of Systematic and Evolutionary Microbiology·Stefan DrögeHelmut König
Mar 3, 2011·Cellular and Molecular Life Sciences : CMLS·Yuichi Hongoh
Mar 27, 2003·Applied Microbiology and Biotechnology·M Ohkuma
Dec 27, 2002·The Journal of General and Applied Microbiology·Jorge W. Santo Domingo
Feb 24, 2001·Environmental Microbiology·T G LilburnJ A Breznak
Mar 3, 2009·Journal of Pediatric Gastroenterology and Nutrition·Shirajum MoniraJehan-Francois Desjeux
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Aug 1, 2013·Applied Microbiology and Biotechnology·Helmut KönigJürgen Fröhlich
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