Physeal bystander effects in rhabdomyosarcoma radiotherapy: experiments in a new xenograft model

Jason A HortonTimothy A Damron


Radiotherapy used in the treatment of pediatric musculoskeletal sarcomas may result in crippling defects of skeletal growth. Several radioprotective strategies have shown potential for preserving function of the irradiated epiphysis but have not been evaluated in a tumor-bearing animal model. We developed two bioluminescent human rhabdomyosarcoma cell lines that were used to establish xenograft tumors in skeletally immature mice. Bioluminescence imaging and radiography allowed serial evaluation of tumor growth and tibial elongation following localized radiotherapy. High-dose (10 Gy) radiotherapy significantly reduced tumor growth velocity and prolonged the median survival of tumor-bearing mice but also resulted in a significant 3.3% shortening of the irradiated limb. Exposure to a lower, 2 Gy dose resulted in 4.1% decrease in limb length but did not extend survival. This new model provides a clinically relevant means to test the efficacy and safety of novel radioprotectant and radiorecovery strategies for use in this context.


Mar 1, 1991·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·J W Smith
May 1, 1997·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·K M KellyF G Barr
Dec 9, 1998·International Journal of Radiation Oncology, Biology, Physics·P VaupelO Thews
May 11, 1999·Hematological Oncology·C F Hoyle, R S Negrin
Jul 19, 2001·Journal of the National Cancer Institute·I J Fidler
Aug 4, 2001·Journal of Clinical Neuroscience : Official Journal of the Neurosurgical Society of Australasia·L WhiteV Tobias
Oct 13, 2001·International Journal of Radiation Oncology, Biology, Physics·S S DonaldsonChildren's Oncology Group Soft Tissue Sarcoma Committee (formely Intergroup Rhabdomyosarcoma Group) representing the Childre
Jul 10, 2003·The Journal of Bone and Joint Surgery. American Volume·Timothy A DamronCornelia E Farnum
Jan 10, 2004·Clinical & Experimental Metastasis·Darlene E JenkinsPamela R Contag
Jul 13, 2004·International Journal of Radiation Biology·Timothy A DamronCornelia E Farnum
Jul 9, 2005·Cellular and Molecular Life Sciences : CMLS·M KolevaH Hahn
Feb 2, 2006·International Journal of Radiation Biology·Joseph A SpadaroTimothy A Damron
Jun 9, 2006·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·M V CéspedesR Mangues
Jul 26, 2006·Journal of Pediatric Surgery·Guido SeitzJohannes T Wessels
Mar 14, 2007·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Peter W T PistersRobert G Maki
Dec 7, 2007·Paediatric Drugs·David Walterhouse, Andrea Watson
Jul 1, 2008·Journal of Neuroscience Methods·Leatha A DamronBrian C Clark
May 1, 2009·Journal of Visualized Experiments : JoVE·Ed LimJaebeom Kim
Jun 2, 2009·International Journal of Radiation Oncology, Biology, Physics·Timothy A DamronJoseph A Spadaro
Aug 18, 2009·Methods in Molecular Biology·Elisa MicheliniAldo Roda

Related Concepts

Epiphysis Disorders
Skeletal System
Xenograft Model
Limb Structure
Bioluminescence Test
Epiphysis of Bone
Bystander Effect

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Synapse Loss as Therapeutic Target in MS

As we age, the number of synapses present in the human brain starts to decline, but in neurodegenerative diseases this occurs at an accelerated rate. In MS, it has been shown that there is a reduction in synaptic density, which presents a potential target for treatment. Here is the latest research on synapse loss as a therapeutic target in MS.

Artificial Intelligence in Cardiac Imaging

Artificial intelligence (ai) techniques are increasingly applied to cardiovascular (cv) medicine in cardiac imaging analysis. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

Social Learning

Social learning involves learning new behaviors through observation, imitation and modeling. Follow this feed to stay up to date on the latest research.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Single Cell Chromatin Profiling

Techniques like ATAC-seq and CUT&Tag have the potential to allow single cell profiling of chromatin accessibility, histones, and TFs. This will provide novel insight into cellular heterogeneity and cell states. Discover the latest research on single cell chromatin profiling here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells.