Physical adsorption of PEG grafted and blocked poly-L-lysine copolymers on adenovirus surface for enhanced gene transduction

Journal of Controlled Release : Official Journal of the Controlled Release Society
Ji Won ParkTae Gwan Park

Abstract

Polyethylene glycol (PEG) has been chemically immobilized onto the surface of adenoviruses (ADVs) to reduce non-specific immune response and extend blood circulation time while maintaining the high transduction efficiency of a foreign gene into cells. In this study, ADVs encoding an exogenous green fluorescent protein (GFP) were physically coated with PEG grafted and blocked poly-L-lysine (PLL-g-PEG and PLL-b-PEG) copolymers via ionic interactions to comparatively evaluate their gene transduction efficiency. The surface immobilization of ADVs with the two types of PLL-PEG copolymers exhibited significantly increased GFP transduction activity, compared to that of naked ADVs. ADVs coated with PLL-b-PEG showed higher extent of GFP expression than those with PLL-g-PEG under serum conditions. For PLL-g-PEG copolymers, the substitution degree of PEG in the PLL backbone greatly influenced the gene expression level. Additionally, ADVs modified with PLL-b-PEG exhibited greater transduction efficiency for bone marrow derived human mesenchymal stem cells compared to naked or PLL coated ADVs in the serum condition. This study suggests that enhanced ADV gene transduction efficiency can be attained for various cells by simply coating PLL-b-P...Continue Reading

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Citations

Dec 31, 2011·Pharmaceutical Research·Martina AntonChristian Plank
Sep 11, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Jun Sung Kang, T Andrew Taton
Oct 26, 2013·Nanomedicine·Seog-Jin SeoHae-Won Kim
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Jul 10, 2012·Nanomedicine : Nanotechnology, Biology, and Medicine·Zibin GaoYongjun Sun
Jan 17, 2012·Colloids and Surfaces. B, Biointerfaces·Yung-Chih Kuo, Chiu-Yen Chung
Jun 28, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society·Oh-Joon KwonChae-Ok Yun
Apr 29, 2011·Journal of Colloid and Interface Science·Stéphanie LouguetChristophe Schatz
Mar 27, 2010·Journal of Controlled Release : Official Journal of the Controlled Release Society·Shyh-Dar Li, Leaf Huang
Jan 26, 2010·Journal of Controlled Release : Official Journal of the Controlled Release Society·Kinam Park
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Feb 25, 2021·Journal of Controlled Release : Official Journal of the Controlled Release Society·Dayananda KasalaChae-Ok Yun

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