Physical and chemical properties of pyropheophorbide-a methyl ester in ethanol, phosphate buffer and aqueous dispersion of small unilamellar dimyristoyl-L-alpha-phosphatidylcholine vesicles

Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology
Lisiane DelanayeMaryse Hoebeke

Abstract

The aggregation process of pyropheophorbide-a methyl ester (PPME), a second-generation photosensitizer, was investigated in various solvents. Absorption and fluorescence spectra showed that the photosensitizer was under a monomeric form in ethanol as well as in dimyristoyl-L-alpha-phosphatidylcholine liposomes while it was strongly aggregated in phosphate buffer. A quantitative determination of reactive oxygen species production by PPME in these solvents has been undertaken by electron spin resonance associated with spin trapping technique and absorption spectroscopy. In phosphate buffer, both electron spin resonance and absorption measurements led to the conclusion that singlet oxygen production was not detectable while hydroxyl radical production was very weak. In liposomes and ethanol, singlet oxygen and hydroxyl radical production increased highly; the singlet oxygen quantum yield was determined to be 0.2 in ethanol and 0.13 in liposomes. The hydroxyl radical production origin was also investigated. Singlet oxygen was formed from PPME triplet state deactivation in the presence of oxygen. Indeed, the triplet state formation quantum yield of PPME was found to be about 0.23 in ethanol, 0.15 in liposomes (too small to be measur...Continue Reading

References

Jan 1, 1992·Photochemistry and Photobiology·B W Henderson, T J Dougherty
Apr 1, 1992·Photochemistry and Photobiology·A Baker, J R Kanofsky
Nov 1, 1991·Photochemistry and Photobiology·C S Foote
Jun 1, 1991·Journal of Photochemistry and Photobiology. B, Biology·M HoebekeA van de Vorst
Jan 1, 1991·Photochemistry and Photobiology·J R Kanofsky
Jun 1, 1991·Radiation Research·T H FosterR Hilf
Apr 15, 1990·Journal of Photochemistry and Photobiology. B, Biology·A A KrasnovskyT Levald
Jun 13, 1986·Biochimica Et Biophysica Acta·L D MayerP R Cullis
Nov 1, 1996·Journal of Photochemistry and Photobiology. B, Biology·G Jori
Mar 4, 1998·Lasers in Surgery and Medicine·F JiangM Chopp
Aug 25, 1999·Drugs & Aging·J S McCaughan
Jun 3, 2000·Journal of Photochemistry and Photobiology. B, Biology·I EichwurzelB Röder
Dec 5, 2002·Photochemistry and Photobiology·Xavier DamoiseauMarie-Pierre Fontaine-Aupart
Mar 29, 2003·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Maryse Hoebeke, Xavier Damoiseau
Dec 23, 1961·Biochimica Et Biophysica Acta·S S BRODY, M BRODY

❮ Previous
Next ❯

Citations

Jul 28, 2011·Photochemistry and Photobiology·Adaickapillai MahendranAlexander Greer
Nov 4, 2008·Expert Opinion on Drug Delivery·Stéphane A SibaniRyan F Donnelly
May 26, 2015·Acta Biomaterialia·Xiaolei ZhouMingdong Huang
Dec 1, 2012·Macromolecular Bioscience·Marie HurtgenChristophe Detrembleur
Jun 10, 2010·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Ioanna StamatiMahendra P Deonarain
Aug 18, 2010·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Pierre-Henri GuelluyMaryse Hoebeke
Jul 1, 2010·Journal of Ultrasound in Medicine : Official Journal of the American Institute of Ultrasound in Medicine·Jing XuAlbert Wingnang Leung
Sep 5, 2020·The Journal of Physical Chemistry Letters·Elena MeneghinElisabetta Collini
Aug 27, 2009·The Journal of Physical Chemistry. B·Jonathan F LovellGang Zheng

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.