Physical and kinematic properties of cryopreserved camel sperm after elimination of semen viscosity by different techniques

Animal Reproduction Science
Khalid El-BahrawyGiovanni Michele Lacalandra

Abstract

This investigation aimed to determine the influence of using different techniques for liquefaction of semen on post-thaw physical and dynamic characteristics of camel spermatozoa. A total of 144 ejaculates were collected from 3 adult camels, Camelus dromedarius, twice-weekly over 3 consecutive breeding seasons. A raw aliquot of each ejaculate was evaluated for physical and morphological properties, whereas the remaining portion was diluted (1:3) with glycerolated Tris lactose egg yolk extender, and was further subjected to one of the following liquefaction treatments: control (untreated), 5μl/ml α-amylase, 0.1mg/ml papain, 5u/ml bromelain, or 40-kHz nominal ultrasound frequency. The post-thaw objective assessment of cryopreserved spermatozoa, in all groups, was performed by a computer-assisted sperm analysis (CASA) system. The results revealed that all liquefaction treatments improved (P<0.05) post-thaw motility, viability and sperm motion criteria. However, an adverse effect (P<0.05) was observed in acrosome integrity, sperm cell membrane integrity and percent of normal sperm in all enzymatically-treated specimens compared to both control and ultrasound-treated semen. These results elucidate the efficiency of utilizing ultraso...Continue Reading

Citations

Jul 26, 2018·Reproduction in Domestic Animals = Zuchthygiene·Aydee MezaCarles Soler
Nov 23, 2019·Animals : an Open Access Journal From MDPI·Ayman Abdel-Aziz SwelumHany Abdalla
Jul 6, 2021·Veterinary World·Ghadeer Sabah Bustani, Falah Hasan Baiee

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