PMID: 8947465Nov 15, 1996Paper

Physical and kinetic characterization of recombinant human cholesteryl ester transfer protein

The Biochemical Journal
D T ConnollyK Glenn

Abstract

Cholesteryl ester transfer protein (CETP) mediates the exchange of triglycerides (TGs), cholesteryl esters (CEs) and phospholipids (PLs) between lipoproteins in the plasma. In order to better understand the lipid transfer process, we have used recombinant human CETP expressed in cultured mammalian cells, purified to homogeneity by immunoaffinity chromatography. Purified recombinant CETP had a weight-average relative molecular mass (MW) of 69561, determined by sedimentation equilibrium, and a specific absorption coefficient of 0.83 litre.g-1.cm-1. The corresponding hydrodynamic diameter (Dh) of the protein, determined by dynamic light scattering, was 14 nm, which is nearly twice the expected value for a spheroidal protein of this molecular mass. These data suggest that CETP has a non-spheroidal shape in solution. The secondary structure of CETP was estimated by CD to contain 32% alpha-helix, 35% beta-sheet, 17% turn and 16% random coil. Like the natural protein from plasma, the recombinant protein consisted of several glycoforms that could be only partially deglycosylated using N-glycosidase F. Organic extraction of CETP followed by TLC showed that CE, unesterified cholesterol (UC), PL, TG and fatty acids (FA) were associated wi...Continue Reading

Citations

Feb 16, 2016·Journal of Structural Biology·Matthias E LauerHenning Stahlberg
Jan 24, 2007·Nature Structural & Molecular Biology·Xiayang QiuAndrew P Seddon
Mar 14, 2009·Journal of Lipid Research·Laura K PotterFrank L Tobin
Jul 27, 2011·Biochemical Society Transactions·Justin Hall, Xiayang Qiu
Aug 24, 2001·Journal of Experimental Psychology. Human Perception and Performance·J KawaharaJ T Enns
Feb 6, 2007·Nature Structural & Molecular Biology·James A Hamilton, Richard J Deckelbaum
Mar 15, 2001·Journal of Applied Physiology·T R ThomasR H Cox
Jul 25, 2017·Scientific Reports·Venkat R Chirasani, Sanjib Senapati
Aug 23, 2002·Journal of Medicinal Chemistry·Richard C DurleyJames A Sikorski

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