Physical association of eukaryotic initiation factor (eIF) 5 carboxyl-terminal domain with the lysine-rich eIF2beta segment strongly enhances its binding to eIF3

The Journal of Biological Chemistry
Chingakham Ranjit SinghK Asano

Abstract

The carboxyl-terminal domain (CTD) of eukaryotic initiation factor (eIF) 5 interacts with eIF1, eIF2beta, and eIF3c, thereby mediating formation of the multifactor complex (MFC), an important intermediate for the 43 S preinitiation complex assembly. Here we demonstrate in vitro formation of a nearly stoichiometric quaternary complex containing eIF1 and the minimal segments of eIF2beta, eIF3c, and eIF5. In vivo, overexpression of eIF2 and tRNA(Met)(i) suppresses the temperature-sensitive phenotype of tif5-7A altering eIF5-CTD by increasing interaction of the mutant eIF5 with eIF2 by mass action and restoring its defective interaction with eIF3. By contrast, overexpression of eIF1 exacerbated the tif5-7A phenotype because eIF1 forms unusual inhibitory complexes with a hyperstoichiometric amount of eIF1. Formation of such complexes leads to increased GCN4 translation, independent of eIF2 phosphorylation (general control derepressed or Gcd(-) phenotype). We also provide biochemical evidence indicating that the association of eIF5-CTD with eIF2beta strongly enhances its binding to eIF3c. Our results suggest strongly that MFC formation is an ordered event involving specific enhancement of eIF5-CTD binding to eIF3 on its binding to eI...Continue Reading

References

Jun 1, 1991·Molecular and Cellular Biology·A M CiganA G Hinnebusch
Nov 28, 2000·The Journal of Biological Chemistry·S DasU Maitra
May 24, 2001·Proceedings of the National Academy of Sciences of the United States of America·P ZhouG Wagner
Jul 11, 2003·Nature·Balázs PappLaurence D Hurst
Dec 12, 2003·Nature·Drew Endy, Michael B Yaffe

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Citations

Oct 29, 2005·Proceedings of the National Academy of Sciences of the United States of America·Yasufumi YamamotoKatsura Asano
Feb 7, 2014·Annual Review of Biochemistry·Alan G Hinnebusch
Mar 23, 2010·Yeast·Tao YouAlistair J P Brown
Jan 1, 2014·Translation·Katsura Asano
Oct 5, 2007·Methods in Enzymology·Chingakham Ranjit Singh, Katsura Asano
Nov 18, 2005·Biochemical and Biophysical Research Communications·Rajasekhar N V S SuraganiKolluru V A Ramaiah
Sep 23, 2006·The EMBO Journal·Chingakham Ranjit SinghKatsura Asano
May 18, 2016·Genetics·Thomas E DeverGraham D Pavitt
Nov 13, 2012·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Hui ZhaoXu Li
Jun 12, 2019·Cancer Investigation·Jianxin HuYoulin Kuang
Nov 18, 2005·Protein Expression and Purification·Rajasekhar N V S SuraganiKolluru V A Ramaiah
Jul 22, 2008·Biochemical and Biophysical Research Communications·Kamindla RajeshKolluru V A Ramaiah

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