Physical, biologic, and phenotypic properties of natural regulatory cells in murine bone marrow

The American Journal of Anatomy
K Dorshkind, C Rosse

Abstract

A lymphocyte-enriched fraction of murine bone marrow (BML) contains natural regulatory cells (NRC) that can inhibit, on a dose-dependent basis, proliferative and cytotoxic responses to alloantigens in a mixed lymphocyte culture. The objective of this study was to investigate the characteristics of the cells responsible for this phenomenon in CBA mice. Maximal suppression was obtained with BML cells themselves rather than cell products. Light-scatter analysis of NRC on the fluorescence-activated cell sorter demonstrated them to be larger than small lymphocytes, and their sedimentation in discontinuous Percoll gradients showed the cells to be of heterogeneous density. This heterogeneity is further reflected by the fact that both plastic adherent and nonadherent BML are suppressive. NRC must be viable in order to mediate suppression; they are cortisone-resistant and are not affected by doses of gamma irradiation up to 1,000 R. NRC are not T or B lymphocytes or Ia-bearing macrophages. The involvement of mature granulocytes and macrophages in natural suppression is unlikely in that NRC do not bear Fc receptors. Elimination of cells from BML with the natural killer (NK) surface marker Asialo GM1 does not abrogate suppression. NRC are...Continue Reading

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Citations

Apr 29, 1998·Stem Cells·K SugiuraS Ikehara
Jul 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·K SugiuraS Ikehara
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Related Concepts

Adrenal Cortex Hormones
Marrow
Bone Marrow Cells
Cell Density
Lymphocyte Culture Test, Mixed
Major Histocompatibility Complex
Mice, Inbred BALB C
Mice, Inbred CBA
Mice, Inbred C3H
Mice, Inbred C57BL

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