Physical crosslinking of collagen fibers: comparison of ultraviolet irradiation and dehydrothermal treatment

Journal of Biomedical Materials Research
K S WeadockM G Dunn

Abstract

The strength, resorption rate, and biocompatibility of collagenous biomaterials are profoundly influenced by the method and extent of crosslinking. We compared the effects of two physical crosslinking methods, ultraviolet irradiation (UV) (254 nm) and dehydrothermal (DHT) treatment, on the mechanical properties and molecular integrity of collagen fibers extruded from an acidic dispersion of type I bovine dermal collagen. Collagen fibers exposed to UV irradiation for 15 min had ultimate tensile strength (54 MPa) and modulus (184 MPa) values greater than or equivalent to values for fibers crosslinked with DHT treatment for 3 or 5 days. UV irradiation is a rapid and easily controlled means of increasing the mechanical strength of collagen fibers. Characterization of collagen extracted from the crosslinked samples by dilute acetic acid and limited pepsin digestion indicate that both UV and DHT treatments cause fragmentation of at least a portion of the collagen molecules. Partial loss of the native collagen structure may influence attachment migration, and proliferation of cells on collagen fiberbased ligament analogs. These issues are currently being addressed in our laboratory.

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Citations

Oct 1, 1996·Journal of Biomedical Materials Research·K S WeadockM G Dunn
Jul 1, 1997·Journal of Biomedical Materials Research·S NehrerM Spector
Jun 24, 1999·Journal of Biomedical Materials Research·H UludagJ Wozney
Mar 29, 2002·Journal of Biomedical Materials Research·Mark P OhanMichael G Dunn
Nov 1, 1995·Journal of Biomedical Materials Research·M G DunnJ P Zawadsky
Sep 24, 2004·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Ronald DorotkaStefan Nehrer
Feb 6, 2007·Journal of Materials Science. Materials in Medicine·Shunji YunokiMasanobu Munekata
Nov 7, 2008·Journal of Materials Science. Materials in Medicine·Markus Dietmar SchoferSusanne Fuchs-Winkelmann
Oct 24, 2009·Journal of Materials Science. Materials in Medicine·Robin S WaltonJan T Czernuszka
Apr 5, 2011·Journal of Materials Science. Materials in Medicine·R A PerezM Spector
Oct 12, 2012·Journal of Materials Science. Materials in Medicine·Eleni Panas-PerezMichael G Dunn
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Aug 17, 2002·Biomaterials·Gregory H AltmanDavid L Kaplan
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