Physical dissection of the structural elements responsible for regulatory properties and intersubunit interactions of protein kinase CK2 beta-subunit

Biochemistry
O MarinLorenzo A Pinna

Abstract

The noncatalytic beta-subunit of protein kinase CK2 has been shown to display various and in some respects antagonistic effects on the catalytic alpha-subunit [Boldyreff et al. (1993) Biochemistry 32, 12672-12677; Meggio et al. (1994) Biochemistry 33, 4336-4342]. We have now examined the ability of peptides encompassing the N- and C-terminal regions of the beta-subunit (beta[1-77] and beta[155-215]) to mimic the functions of the whole-length beta-subunit. Peptide beta[155-215] possesses only the positive features of the beta-subunit in that it prevents thermal inactivation and stimulates basal activity of the alpha-subunit, while it does not inhibit but rather stimulates calmodulin phosphorylation. In sharp contrast, peptide beta[1-77] neither protects the alpha-subunit nor stimulates its basal activity, while acting as a powerful and specific inhibitor of calmodulin phosphorylation. Peptide beta[155-215], but not peptide beta[1-77], stably interacts with alpha-subunit and also displays remarkable self-associating properties. A shorter derivative of beta[155-215], beta[170-215], displaying weaker stimulatory properties fails to stably interact with the alpha-subunit and to give rise to dimeric/multimeric forms. These data show ...Continue Reading

References

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Citations

Sep 5, 2002·Biochemical and Biophysical Research Communications·Sonia FacchinLorenzo A Pinna
Aug 24, 1999·Pharmacology & Therapeutics·B GuerraL A Pinna
Feb 5, 2000·Progress in Neurobiology·P R Blanquet
Nov 4, 2000·The Biochemical Journal·F MeggioL A Pinna
Apr 9, 2008·Proceedings of the National Academy of Sciences of the United States of America·Fernando A RodriguezJorge E Allende
Aug 20, 2003·Proceedings of the National Academy of Sciences of the United States of America·Oriano MarinJorge E Allende
Jun 17, 2006·Biochemical and Biophysical Research Communications·Paola CoccettiLilia Alberghina
Jun 17, 2011·The Biochemical Journal·Ratnesh K SrivastavRainer Niedenthal
Jun 25, 1999·Biochemical and Biophysical Research Communications·F PancettiO Bachs
Jan 19, 2019·Journal of Human Genetics·Mitsuko NakashimaNaomichi Matsumoto
May 5, 2005·The Journal of Clinical Endocrinology and Metabolism·Claudio PaganoRoberto Vettor
Aug 8, 2007·The Journal of Biological Chemistry·Ashley C FrenchDavid W Litchfield
Feb 15, 2000·The Journal of Biological Chemistry·K C Graham, D W Litchfield
Aug 24, 2007·The Biochemical Journal·Béatrice LaudetClaude Cochet
Dec 10, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Vincenzo BrontePaola Zanovello
Dec 24, 2018·The Biochemical Journal·Antonio Villalobo

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