Physical Exercise Enhanced Heat Shock Protein 60 Expression and Attenuated Inflammation in the Adipose Tissue of Human Diabetic Obese

Frontiers in Endocrinology
Abdelkrim KhadirAli Tiss

Abstract

Heat shock protein 60 (HSP60) is a key protein in the crosstalk between cellular stress and inflammation. However, the status of HSP60 in diabetes and obesity is unclear. In the present study, we investigated the hypothesis that HSP60 expression levels in the adipose tissue of human obese adults with and without diabetes are different and physical exercise might affect these levels. Subcutaneous adipose tissue (SAT) and blood samples were collected from obese adults with and without diabetes (n = 138 andn = 92, respectively, at baseline;n = 43 for both groups after 3 months of physical exercise). Conventional RT-PCR, immunohistochemistry, immunofluorescence, and ELISA were used to assess the expression and secretion of HSP60. Compared with obese adults without diabetes, HSP60 mRNA and protein levels were decreased in SAT in diabetic obese together with increased inflammatory marker expression and glycemic levels but lower VO2 Max. More interestingly, a 3-month physical exercise differentially affected HSP60 expression and the heat shock response but attenuated inflammation in both groups, as reflected by decreased endogenous levels of IL-6 and TNF-α. Indeed, HSP60 expression levels in SAT were significantly increased by exercis...Continue Reading

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Citations

Apr 11, 2020·Cell Communication and Signaling : CCS·Deling ZhangChanghua Wang
Mar 28, 2020·Cell Stress & Chaperones·Yana Picinin Sandri LissarassaMirna Stela Ludwig
Dec 24, 2018·Lipids in Health and Disease·Abdelkrim KhadirAli Tiss
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Mar 21, 2020·International Journal of Molecular Sciences·Abdelkrim KhadirAli Tiss
Oct 9, 2020·Computational and Mathematical Methods in Medicine·Xiao-Yang Jing, Feng-Min Li
Mar 10, 2021·Cardiovascular Drugs and Therapy·Sophie N SaxtonAnthony M Heagerty

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Methods Mentioned

BETA
biopsies
biopsy
ELISA
sandwich immunoassay
PCR

Software Mentioned

ImageScope
SPSS

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