Physical interaction between Sit1 and Aft1 upregulates FOB uptake activity by inhibiting protein degradation of Sit1 in Saccharomyces cerevisiae

FEMS Yeast Research
Chang-Min KangCheol-Won Yun

Abstract

Previously, we reported that Aft1 regulates Sit1 by modulating the ubiquitination of Sit1 in Saccharomyces cerevisiae. Here, we report the function of the physical interaction between Sit1 and Aft1 in ferrioxamine B (FOB) uptake. The interaction between Sit1 and Aft1 induced protein localization of Sit1 to the plasma membrane, and more Sit1 was detected in the plasma membrane when Sit1 and Aft1 were coexpressed compared with Sit1 expression alone. The MSN5-deletion mutant, which failed to translocate Aft1 to the cytosolic compartment, showed lower FOB uptake activity than the wild type. However, higher free iron uptake activity was detected in the MSN5-deletion mutant. Furthermore, the strain transformed with AFT1-1(up) plasmid, which failed to regulate Aft1 via iron concentration and accumulated Aft1 in the nucleus, showed lower FOB uptake activity. The Aft1 Y179F mutant, which contained a tyrosine residue that was changed to phenylalanine, failed to interact physically with Sit1 and showed more degradation of the Sit1 and, ultimately, lower FOB uptake activity. Additionally, we found that MG132 and PMSF, which are inhibitors of proteasomes and serine proteases, respectively, increased the Sit1 protein level. Taken together, t...Continue Reading

References

May 1, 1990·Molecular and Cellular Biology·A DancisJ G Barriocanal
Jan 1, 1988·Free Radical Biology & Medicine·R A Kloner
May 19, 2000·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·P HeymannG Winkelmann
Jun 1, 2000·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·P HeymannG Winkelmann
Mar 6, 2002·The Journal of Biological Chemistry·Yuko Yamaguchi-IwaiRyuzo Sasaki
May 21, 2003·The Journal of Biological Chemistry·Julian C RutherfordDennis R Winge
Oct 3, 2003·The Journal of Biological Chemistry·Ryo UetaYuko Yamaguchi-Iwai
Feb 19, 2005·The EMBO Journal·Youngwoo KimCaroline C Philpott
Nov 13, 2007·Eukaryotic Cell·Caroline C Philpott, Olga Protchenko
May 21, 2008·Traffic·Zoi ErpapazoglouNaïma Belgareh-Touzé
Jul 19, 2011·Developmental Cell·William M HenneScott D Emr
Mar 13, 2012·Annual Review of Biochemistry·Jason A MacGurnScott D Emr

❮ Previous
Next ❯

Citations

Feb 22, 2017·Metallomics : Integrated Biometal Science·Gaurav BairwaJames W Kronstad
Jul 15, 2020·Biochimica Et Biophysica Acta. Molecular Cell Research·María Teresa Martínez-Pastor, Sergi Puig

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.